Noninvasive estimation of light scattering and hemoglobin concentration in mice cutaneous carcinogenesis through multispectral imaging

Skin cancer is the most common cancer, predominantly found in people with light-skin color. With a view to diagnosing at early stage, we evaluated a new optical method to investigate the alterations in skin morphology and hemodynamics during skin cancer in mice by multispectral imaging system as traditional measures based on gross lesions that appear at the advanced stage when the prognosis is terrible. Here, the use of isosbestic wavelengths of hemoglobin makes it possible to measure hemoglobin concentration irrespective of oxygenation status of hemoglobin. Results illustrate that while there scattering power decreased, hemoglobin concentration increased in the carcinogenic mice. It demonstrated that the proposed system is competent to monitor pathophysiological changes specially scattering power and hemoglobin concentration during cutaneous two-stage chemical carcinogenesis.