Fluorogenic probes for super-resolution microscopy

被引:87
|
作者
Kozma, Eszter [1 ]
Kele, Peter [1 ]
机构
[1] Hungarian Acad Sci, Inst Organ Chem, Res Ctr Nat Sci, Chem Biol Res Grp, Magyar Tudosok Krt 2, H-1117 Budapest, Hungary
关键词
SINGLE DNA-MOLECULES; LIVE-CELL; FLUORESCENCE NANOSCOPY; INTRAMOLECULAR SPIROCYCLIZATION; PHOTOSWITCHABLE DIARYLETHENES; TETRAZINE PROBES; BACKGROUND-FREE; LIVING CELLS; CYANINE DYES; LOCALIZATION;
D O I
10.1039/c8ob02711k
中图分类号
O62 [有机化学];
学科分类号
070303 ; 081704 ;
摘要
Fluorogenic probes efficiently reduce non-specific background signals, which often results in highly improved signal-to-noise ratios. Although this implies improved resolution, fluorogenic probes in the context of super-resolution microscopy are somewhat overlooked. Several excellent reviews summarize recent developments in SRM techniques, labeling techniques or different aspects of small synthetic fluorophores, however there is no comprehensive review on fluorogenic probes suitable for super-resolution microscopy. Herein we wish to fill this gap by providing the readers with an up-to-date summary of fluorogenic probes applied to super-resolution imaging of cellular structures.
引用
收藏
页码:215 / 233
页数:19
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