Immunosensing by colorimetric darkfield microscopy of individual gold nanoparticle-conjugates

被引：11
|
作者：
Ungureanu, Felicia [1 ]
Wasserberg, Dorothee [1 ]
Yang, Ni [2 ,3 ]
Verdoold, Remco [1 ]
Kooyman, Rob P. H. [1 ]
机构：
[1] Univ Twente, NanoBioPhys Grp, MESA Inst, NL-7522 ND Enschede, Netherlands
[2] Univ Alberta, Dept Chem, Chem Ctr W4 09, Edmonton, AB T6G 2G2, Canada
[3] Natl Inst Nanotechnol Devices & Sensors, Genom Hlth Initiat, Edmonton Res Facil, Edmonton, AB T6G 2M9, Canada
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2010年 / 150卷 / 02期
关键词：
Gold nanoparticles; Colorimetric darkfield microscopy; Immunosensor; Parallel detection; Affinity constant; SURFACE-PLASMON RESONANCE; OPTICAL-PROPERTIES; SILVER NANOPARTICLES; SPECTROSCOPY; DEPENDENCE; BINDING; ABSORPTION; SERUM; SIZE;
D O I：
10.1016/j.snb.2010.08.044
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A new (generic) sensor platform introduced using gold colorimetric data showed that binding events of individual particles could be discerned for about 40% of the available binding sites while reference data showed less than 005% binding Endpoint SEM images of the sample and reference binding couples corroborate our findings (C) 2010 Elsevier B V All rights reserved
引用
收藏
页码：529 / 536
页数：8
相关论文
共 50 条
  • [1] Gold nanoparticles and gold nanoparticle-conjugates for delivery of therapeutic molecules. Progress and challenges
    Fratoddi, I.
    Venditti, I.
    Cametti, C.
    Russo, M. V.
    [J]. JOURNAL OF MATERIALS CHEMISTRY B, 2014, 2 (27) : 4204 - 4220
  • [2] Femtomolar DNA detection by parallel colorimetric darkfield microscopy of functionalized gold nanoparticles
    Verdoold, Remco
    Gill, Ron
    Ungureanu, Felicia
    Molenaar, Robert
    Kooyman, Rob P. N.
    [J]. BIOSENSORS & BIOELECTRONICS, 2011, 27 (01): : 77 - 81
  • [3] Role of Nanoparticle-Conjugates and Nanotheranostics in Abrogating Oxidative Stress and Ameliorating Neuroinflammation
    Patel, Tapan A.
    Kevadiya, Bhavesh D.
    Bajwa, Neha
    Singh, Preet Amol
    Zheng, Hong
    Kirabo, Annet
    Li, Yu-Long
    Patel, Kaushik P.
    [J]. ANTIOXIDANTS, 2023, 12 (10)
  • [4] In-Situ Conjugation - Tailored Nanoparticle-Conjugates by Laser Ablation in Liquids
    Petersen, Svea
    Jakobi, Jurij
    Hoertinger, Angelika
    Barcikowski, Stephan
    [J]. JOURNAL OF LASER MICRO NANOENGINEERING, 2009, 4 (01): : 71 - 74
  • [5] Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy
    Ge, Feng
    Xue, Jianfeng
    He, Yan
    [J]. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2021, (169):
  • [6] Colorimetric detection with aptamer–gold nanoparticle conjugates: effect of aptamer length on response
    Jorge L. Chávez
    Robert I. MacCuspie
    Morley O. Stone
    Nancy Kelley-Loughnane
    [J]. Journal of Nanoparticle Research, 2012, 14
  • [7] Colorimetric immunosensing via protein functionalized gold nanoparticle probe combined with atom transfer radical polymerization
    Shi, Hongyan
    Yuan, Liang
    Wu, Yafeng
    Liu, Songqin
    [J]. BIOSENSORS & BIOELECTRONICS, 2011, 26 (09): : 3788 - 3793
  • [8] In situ bioconjugation-Novel laser based approach to pure nanoparticle-conjugates
    Petersen, Svea
    Jakobi, Jurij
    Barcikowski, Stephan
    [J]. APPLIED SURFACE SCIENCE, 2009, 255 (10) : 5435 - 5438
  • [9] Use of aptamer-gold nanoparticle conjugates to develop colorimetric sensors for clinical application
    Haines, Robert A.
    Ozer, Ruya R.
    [J]. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2011, 241
  • [10] Colorimetric detection with aptamer-gold nanoparticle conjugates: effect of aptamer length on response
    Chavez, Jorge L.
    MacCuspie, Robert I.
    Stone, Morley O.
    Kelley-Loughnane, Nancy
    [J]. JOURNAL OF NANOPARTICLE RESEARCH, 2012, 14 (10)
12345