Live confocal imaging of Arabidopsis flower buds

被引：34
|
作者：
Prunet, Nathanael [1 ]
Jack, Thomas P. [2 ]
Meyerowitz, Elliot M. [1 ,3 ]
机构：
[1] CALTECH, Div Biol & Biol Engn, 1200 E Calif Blvd, Pasadena, CA 91125 USA
[2] Dartmouth Coll, Dept Biol Sci, 78 North Coll St, Hanover, NH 03755 USA
[3] CALTECH, Howard Hughes Med Inst, 1200 E Calif Blvd, Pasadena, CA 91125 USA
来源：
DEVELOPMENTAL BIOLOGY | 2016年 / 419卷 / 01期
基金：
美国国家科学基金会; 美国国家卫生研究院;
关键词：
Flower; Flower development; Flower meristem; Plant development; Confocal microscopy; Live confocal imaging; Sepals; Floral organs; FLORAL DEVELOPMENT; SHOOT APEX; THALIANA; EXPRESSION; CELLS; GENE;
D O I：
10.1016/j.ydbio.2016.03.018
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Recent advances in confocal microscopy, coupled with the development of numerous fluorescent reporters, provide us with a powerful tool to study the development of plants. Live confocal imaging has been used extensively to further our understanding of the mechanisms underlying the formation of roots, shoots and leaves. However, it has not been widely applied to flowers, partly because of specific challenges associated with the imaging of flower buds. Here, we describe how to prepare and grow shoot apices of Arabidopsis in vitro, to perform both single-point and time-lapse imaging of live, developing flower buds with either an upright or an inverted confocal microscope. (C) 2016 Elsevier Inc. All rights reserved.
引用
收藏
页码：114 / 120
页数：7
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