Differentiation of functional astrocytes from human- induced pluripotent stem cells in chemically defined media

被引：13
|
作者：
Perriot, Sylvain [1 ,2 ]
Canales, Mathieu [1 ,2 ]
Mathias, Amandine [1 ,2 ]
Du Pasquier, Renaud [1 ,2 ,3 ]
机构：
[1] Univ Lausanne, Lausanne Univ Hosp CHUV, Lab Neuroimmounol Neurosci Res Ctr, Dept Clin Neurosci, CH-1015 Lausanne, Switzerland
[2] Univ Lausanne, CH-1015 Lausanne, Switzerland
[3] Univ Lausanne, Lausanne Univ Hosp CHUV, Dept Clin Neurosci, Serv Neurol, CH-1015 Lausanne, Switzerland
来源：
STAR PROTOCOLS | 2021年 / 2卷 / 04期
基金：
瑞士国家科学基金会;
关键词：
Cell Biology; Cell culture; Cell Differentiation; Neuroscience; Stem Cells;
D O I：
10.1016/j.xpro.2021.100902
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This protocol describes how to obtain human astrocytes from human-induced pluripotent stem cells (hiPSCs) in chemically defined media, without the use of fetal bovine serum (FBS). FBS eases the differentiation of astrocytes but also deeply alters their phenotype, as compared with their in vivo counterparts. Our protocol generates hiPSC-derived astrocytes displaying a phenotype and functions similar to human primary astrocytes, including adequate response to inflammation, neurotransmitter uptake, and trophic support to neurons. For complete details on the use and execution of this protocol, please refer to Perriot et al. (2018).
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收藏
页数：13
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