Simultaneous synthesis of luciferase and human nerve growth factor in caterpillars infected with a recombinant baculovirus

被引：0

作者：

Zhang, XW ^{[1
]}

Sun, T ^{[1
]}

Liu, X ^{[1
]}

Huang, XN ^{[1
]}

机构：

[1] Zhongshan Univ, State Educ Commis, Food Engn Res Ctr, Guangzhou 510275, Peoples R China

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | 1998年 / 21卷 / 06期

关键词：

hNGF; Luc; PCR; baculovirus system; transfer vector; gene expression;

D O I：

10.1038/sj.jim.2900579

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Human nerve growth factor (hNGF) gene was proliferated with human leucocyte DNA as template by PCR. Then a fusion gene coding hNGF and luciferase (Luc) cDNAs was inserted into transfer vector pSXIVVI(+)X3/3 with the control of Syn XIV promoter. Luc and hNGF were simultaneously synthesized in Spodoptera larvae upon infection with a recombinant baculovirus, TnNPV-Luc-NGF-OCC+. Densitometric scanning of SDS-PAGE revealed that similar to 18% of the total Coomassie blue-stainable protein of the infected larvae was represented by Luc protein, while the hNGF lever was similar to 8%. Both proteins were similar to their authentic counterparts in terms of immunoreactivity.

引用

页码：289 / 291

页数：3

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