Study on naringenin-CTMAB-DNA system by resonance light scattering technique and its analytical application

被引：7
|
作者：
Bi, Shuyun [1 ]
Wang, Yu [1 ]
Pang, Bo [1 ]
Yan, Lili [1 ]
机构：
[1] Changchun Normal Univ, Coll Chem, Changchun 130032, Peoples R China
来源：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2011年 / 79卷 / 05期
关键词：
Resonance light scattering (RLS); Naringenin; CTMAB; DNA; CETYLTRIMETHYLAMMONIUM BROMIDE SYSTEM; NUCLEIC-ACIDS; NANOGRAM LEVELS; FLUOROMETRIC-DETERMINATION; DEOXYRIBONUCLEIC-ACID; SPECTRUM; COMPLEX; PROBE;
D O I：
10.1016/j.saa.2011.04.081
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
A new high-sensitivity determination method of deoxyribonucleic acid (DNA) with detection limit at nanogram levels was proposed. Based on the measurement of resonance light scattering (RLS), it was found DNA could combine with naringenin and cetyltrimethylammonium bromide (CTMAB) in basic Tris-HCI buffer and produce enhanced RLS signal. The optimum conditions for this system were studied in detail. The enhanced intensity of RLS of naringenin-CTMAB at 353 nm was directly proportional to the concentration of DNA in the range of 0.017-1.7 mu g mL(-1). The detection limit was 5.06 ng mL(-1). Using the proposed method, the synthetic samples were analyzed with satisfactory results, the recovery was 99.3-105.0% and RSD was 0.7-3.7%. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：1430 / 1434
页数：5
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