Interbacterial signaling via Burkholderia contact-dependent growth inhibition system proteins

被引:74
|
作者
Garcia, Erin C. [1 ]
Perault, Andrew I. [1 ]
Marlatt, Sara A. [1 ]
Cotter, Peggy A. [1 ]
机构
[1] Univ N Carolina, Dept Microbiol & Immunol, Chapel Hill, NC 27599 USA
基金
美国国家卫生研究院;
关键词
signal transduction; sociomicrobiology; contact-dependent competition; two-partner secretion; biofilm; C-DI-GMP; BIOFILM FORMATION; ESCHERICHIA-COLI; INNER MEMBRANE; IDENTIFICATION; COMMUNICATION; CONTRIBUTES; EXPRESSION; BACTERIA; RECEPTOR;
D O I
10.1073/pnas.1606323113
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
In prokaryotes and eukaryotes, cell-cell communication and recognition of self are critical to coordinate multicellular functions. Although kin and kind discrimination are increasingly appreciated to shape naturally occurring microbe populations, the underlying mechanisms that govern these interbacterial interactions are insufficiently understood. Here, we identify a mechanism of interbacterial signal transduction that is mediated by contact-dependent growth inhibition (CDI) system proteins. CDI systems have been characterized by their ability to deliver a polymorphic protein toxin into the cytoplasm of a neighboring bacterium, resulting in growth inhibition or death unless the recipient bacterium produces a corresponding immunity protein. Using the model organism Burkholderia thailandensis, we show that delivery of a catalytically active CDI system toxin to immune (self) bacteria results in gene expression and phenotypic changes within the recipient cells. Termed contact-dependent signaling (CDS), this response promotes biofilm formation and other community-associated behaviors. Engineered strains that are isogenic with B. thailandensis, except the DNA region encoding the toxin and immunity proteins, did not display CDS, whereas a strain of Burkholderia dolosa producing a nearly identical toxin-immunity pair induced signaling in B. thailandensis. Our data indicate that bcpAIOB loci confer dual benefits; they direct antagonism toward non-self bacteria and promote cooperation between self bacteria, with self being defined by the bcpAIOB allele and not by genealogic relatedness.
引用
收藏
页码:8296 / 8301
页数:6
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