Seroprevalence of Campylobacter spp. using enzyme-linked immunosorbent assay in humans and chickens from selected areas of metro manila and Laguna, Philippines

An enzyme-linked immunosorbent assay (ELISA) was adopted to determine the occurrence of antibodies for Campylobacter jejuni/coli and C. jejuni 0: 19 in 128 human and 128 chicken serum samples. Seroprevalence to C. jejuni/coli among humans tested was found very high at 96.1% (123/128), compared to C. jejuni 0: 19 at 0.6% (2/128). This is the first report on C. jejuni/coli seroprevalence in the country. Moreover, it is also the first report for serodiagnosis of C. jejuni 0: 19 in. the Philippines. The prevalence rates for antibodies to C. jejuni/coli and C.jejuni 0: 19 among different age groups and between sexes were not significantly different (P < 0.05). Furthermore, there was also no difference in seroprevalence among exposed (chicken workers) and non-exposed (non-chicken workers) groups for C. jejuni/coli. However, the prevalence rate for C. jejuni 0: 19 antibodies was higher (P < 0. 05) in the non-exposed group. In chickens, antibodies to C.jejuni/coli were also observed to be very high (P<0.01) at 80.5% (103/128) whereas, no antibodies to C. jejuni 0: 19 was detected.