Conjunctival Sac Microbiome in Infectious Conjunctivitis

被引:7
|
作者
Mohamed, Yasser Helmy [1 ]
Uematsu, Masafumi [1 ]
Morinaga, Yoshitomo [2 ]
Nguyen, Hien-Anh Thi [3 ]
Toizumi, Michiko [4 ]
Sasaki, Daisuke [2 ]
Yanagihara, Katsunori [2 ]
Dang, Duc-Anh [3 ]
Kitaoka, Takashi [1 ]
Yoshida, Lay-Myint [4 ]
机构
[1] Nagasaki Univ, Grad Sch Biomed Sci, Dept Ophthalmol & Visual Sci, 1-7-1 Sakamoto, Nagasaki 8528501, Japan
[2] Nagasaki Univ, Grad Sch Biomed Sci, Dept Lab Med, Nagasaki 8528501, Japan
[3] Natl Inst Hyg & Epidemiol, Hanoi 100000, Vietnam
[4] Nagasaki Univ, Inst Trop Med, Dept Pediat Infect Dis, Nagasaki 8528501, Japan
关键词
conjunctival microbiome; infectious conjunctivitis; polymerase chain reaction; 16S ribosomal DNA sequencing; BACTERIAL-FLORA; IDENTIFICATION; PCR; PATHOGENS; EYES;
D O I
10.3390/microorganisms9102095
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Acute bacterial conjunctival infections are common, and this study identified the conjunctival bacterial community in infectious conjunctivitis cases seen at the outpatient clinic of Khanh Hoa General Hospital in Nha Trang, Vietnam from October 2016 through December 2017. Conjunctival swabs were collected and tested using conventional culture, PCR, and 16S ribosomal RNA sequencing. The study included 47 randomly selected patients. More than 98% of all DNA reads represented five bacterial phyla. Three of these phyla constituted 92% of all sequences (Firmicutes (35%), Actinobacteria (31%), and Proteobacteria (26%)). At the genus level, there were 12 common genera that constituted about 61% of all sequence reads. Seven of those genera were common (Streptococcus (10%), Cutibacterium (10%), Staphylococcus (7%), Nocardioides (7%), Corynebacterium 1 (5%), Anoxybacillus (5%), and Acinetobacter (5%)), which encompassed 49% of all reads. As for diversity analysis, there was no difference on PERMANOVA analysis (unweighted UniFrac) for sex (p = 0.087), chemosis (p = 0.064), and unclassified eyedrops (p = 0.431). There was a significant difference in cases with bilateral conjunctivitis (p = 0.017) and for using antibiotics (p = 0.020). Of the predominant phyla, Firmicutes had the highest abundance in bacterial conjunctivitis in this study. Pseudomonas as a resident commensal microbiota may have an important role in the prevention of infection.</p>
引用
收藏
页数:12
相关论文
共 50 条
  • [31] Reconstruction of a conjunctival sac with Thiersch grafts
    Glover, DM
    Jacoby, MW
    ARCHIVES OF SURGERY, 1933, 26 (04) : 617 - 622
  • [32] RESORPTION OF STEROIDS FROM THE CONJUNCTIVAL SAC
    OHMAN, L
    JOHANSSON, EDB
    ACTA ENDOCRINOLOGICA, 1979, 91 : 110 - 110
  • [33] Conjunctival caltoma: a threatening state of chronic conjunctivitis
    Creuzot-Garcher, C
    Bron, A
    JOURNAL FRANCAIS D OPHTALMOLOGIE, 1999, 22 (05): : 581 - 583
  • [34] EYELID OINTMENT PENETRATING INTO CONJUNCTIVAL SAC
    NORN, MS
    ACTA OPHTHALMOLOGICA, 1972, 50 (02): : 206 - &
  • [35] A new pathogenic microorganism of the conjunctival sac
    McKee, H
    JOURNAL OF MEDICAL RESEARCH, 1907, 16 : 527 - 535
  • [36] INFECTIOUS BOVINE KERATO CONJUNCTIVITIS
    BRUGEREPICOUX, J
    RECUEIL DE MEDECINE VETERINAIRE, 1979, 155 (03) : 201 - 209
  • [37] Mimicking Picture of Infectious Conjunctivitis
    Lin, Tzu-Yu
    Peng, Tao-Chun
    AMERICAN JOURNAL OF MEDICINE, 2016, 129 (02): : E51 - E51
  • [38] Infectious etiologies of conjunctival tumors
    Alvarez, Osmel P.
    Monroy, David
    Thura, Soraiya
    Antonietti, Michael
    Fasih-Ahmad, Sohaib
    Sepulveda-Beltran, Paula A.
    Culbertson, Sara
    Dubovy, Sander R.
    Galor, Anat
    Karp, Carol L.
    EXPERT REVIEW OF OPHTHALMOLOGY, 2025, 20 (01) : 3 - 18
  • [39] Ligneous conjunctivitis masquerading as a large, recurrent conjunctival tumor
    Martel, A.
    Nahon-Esteve, S.
    Flory, A. Appert
    Lassalle, S.
    Leonardo, S.
    Tieulie, N.
    Caujolle, J. -P.
    JOURNAL FRANCAIS D OPHTALMOLOGIE, 2021, 44 (03): : 451 - 453
  • [40] Pseudomembranous Conjunctivitis: A Possible Conjunctival Foreign Body Aetiology
    Ho, Dawn
    Lim, Shandy
    Teck, Yeo Kim
    CUREUS JOURNAL OF MEDICAL SCIENCE, 2020, 12 (05)