Silencing of tripartite motif protein (TRIM) 5α mediated anti-HIV-1 activity by truncated mutant of TRIM5α

Tripartite motif protein (TRIM) 5 alpha is a restriction factor of human immunodeficiency virus type I in Old World monkey cell. It was found that both naturally Occurring and artificial TRIM5 alpha variants lacking the SPRY domain Could silence TRIM5 alpha activity. Specifically, the artificial TRIM5 alpha mutant Could Suppress TRIM5 alpha activity of various primate species with even higher efficiency than Could small interfering RNAs. The findings indicate that TRIM5 alpha variants lacking the SPRY domain are useful for silencing TRIM5 alpha activity. (c) 2008 Elsevier B.V. All rights reserved.