Growth of the hyperthermophilic marine Archaeon Aeropyrum pernix in a defined medium

被引:0
|
作者
Kim, KW
Lee, SB
机构
[1] Pohang Univ Sci & Technol, Dept Chem Engn, Pohang 790784, South Korea
[2] Pohang Univ Sci & Technol, Div Mol & Life Sci, Pohang 790784, South Korea
关键词
Aeropyrum pernix; defined medium; hyperthermophile; archaeon; cell growth;
D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The nutritional requirements of Aeropyrum pernix were studied to investigate the growth of a hyperthermophilic marine archaeon in a defined medium. Various nutrients including sugars, amino acids, and nucleoside bases were tested to determine their effects on cell growth. It was found that adenine and six amino acids (Arg, Ile, Leu, Lys, Met, Val) are essential for the growth of A. pernix. An artificial seawater-based medium, containing 11 amino acids, adenine, vitamins, KH2PO4, Na2S2O3, and trace minerals, enabled the growth of A. pernix at a cell density and specific growth rate similar to those obtained with complex media.
引用
收藏
页码:618 / 622
页数:5
相关论文
共 50 条
  • [31] Study on the relationship between structure and enantioselectivity of a hyperthermophilic esterase from archaeon Aeropyrum pernix K1
    Zhang, GR
    Gao, RJ
    Zheng, LY
    Zhang, AJ
    Wang, YH
    Wang, QY
    Feng, Y
    Cao, SG
    JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2006, 38 (3-6) : 148 - 153
  • [32] Enhanced Activity and Enantioselectivity of a Hyperthermophilic Esterase from Archaeon Aeropyrum pernix K1 by Acetone Treatment
    Cong, Fangdi
    Xing, Kezhi
    Gao, Renjun
    Cao, Shugui
    Zhang, Guirong
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2011, 165 (3-4) : 795 - 801
  • [33] Characterization of thermostable native alkaline phosphatase from an aerobic hyperthermophilic archaeon, Aeropyrum pernix K1
    Is Helianti
    Takako Okubo
    Yasutaka Morita
    Eiichi Tamiya
    Applied Microbiology and Biotechnology, 2007, 74 : 107 - 112
  • [34] Crystallization and preliminary crystallographic analysis of acylamino-acid releasing enzyme from the hyperthermophilic archaeon Aeropyrum pernix
    Wang, GG
    Gao, RJ
    Ding, Y
    Yang, HT
    Cao, SG
    Feng, Y
    Rao, ZH
    ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2002, 58 : 1054 - 1055
  • [35] Characterization of thermostable native alkaline phosphatase from an aerobic hyperthermophilic archaeon, Aeropyrum pernix K1
    Helianti, Is
    Okubo, Takako
    Morita, Yasutaka
    Tamiya, Eiichi
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2007, 74 (01) : 107 - 112
  • [36] Enhanced Activity and Enantioselectivity of a Hyperthermophilic Esterase from Archaeon Aeropyrum pernix K1 by Acetone Treatment
    Fangdi Cong
    Kezhi Xing
    Renjun Gao
    Shugui Cao
    Guirong Zhang
    Applied Biochemistry and Biotechnology, 2011, 165 : 795 - 801
  • [37] Biochemical analysis of a DNA replication origin in the archaeon Aeropyrum pernix
    Grainge, Ian
    Gaudier, Martin
    Schuwirth, Barbara S.
    Westcott, Sarah L.
    Sandall, Jane
    Atanassova, Neli
    Wigley, Dale B.
    JOURNAL OF MOLECULAR BIOLOGY, 2006, 363 (02) : 355 - 369
  • [38] Crystal Structure of Novel Dye-linked L-Proline Dehydrogenase from Hyperthermophilic Archaeon Aeropyrum pernix
    Sakuraba, Haruhiko
    Satomura, Takenori
    Kawakami, Ryushi
    Kim, Kwang
    Hara, Yusuke
    Yoneda, Kazunari
    Ohshima, Toshihisa
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2012, 287 (24) : 20070 - 20080
  • [39] Overexpression, purification and crystallization of tyrosyl-tRNA synthetase from the hyperthermophilic archaeon Aeropyrum pernix K1
    Iwaki, J
    Suzuki, R
    Fujimoto, Z
    Momma, M
    Kuno, A
    Hasegawa, T
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2005, 61 : 1003 - 1005
  • [40] Stability of diether C25,25 liposomes from the hyperthermophilic archaeon Aeropyrum pernix K1
    Gmajner, Dejan
    Ota, Ajda
    Sentjurc, Marjeta
    Ulrih, Natasa Poklar
    CHEMISTRY AND PHYSICS OF LIPIDS, 2011, 164 (03) : 236 - 245