Diagnostic potential of toscana virus N protein expressed in Escherichia coli

被引：18
|
作者：
Valassina, M
Soldateschi, D
Dal Maso, GM
Santini, L
Bianchi, S
Valensin, PE
Cusi, MG [1 ]
机构：
[1] Univ Siena, Dept Mol Biol, Microbiol Sect, I-53100 Siena, Italy
[2] DIESSE Srl, I-53035 Monteriggioni, SI, Italy
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 1998年 / 36卷 / 11期
关键词：
D O I：
10.1128/JCM.36.11.3170-3172.1998
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The nucleocapsid (N) protein of the Toscana (TOS) virus was expressed in Escherichia coli by using a pET15b vector. The recombinant protein was purified by affinity chromatography and was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and enzyme immunoassay (EIA), The recombinant antigen was reactive with positive human sera, and the reactivity correlated very well (r = 0.9) with that of a whole-virus antigen when tested by EIA with 30 TOS virus-positive and 30 TOS virus-negative serum samples. The results demonstrate that the recombinant N protein can be easily produced in a procaryotic system and used for diagnostic assays for TOS virus immunity.
引用
收藏
页码：3170 / 3172
页数：3
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