Real-Time PCR Assay for Clostridium perfringens in Broiler Chickens in a Challenge Model of Necrotic Enteritis

被引：31

作者：

Wu, Shu-Biao ^{[1
]}

Rodgers, Nicholas ^{[1
]}

Choct, Mingan ^{[1
]}

机构：

[1] Univ New England, Sch Environm & Rural Sci, Armidale, NSW 2351, Australia

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2011年 / 77卷 / 03期

关键词：

INTESTINAL BACTERIAL COMMUNITY; QUANTITATIVE PCR; POPULATIONS; QUANTIFICATION; IDENTIFICATION; LACTOBACILLUS; ENUMERATION; PATHOGENS; PRIMERS; SAMPLES;

D O I：

10.1128/AEM.01803-10

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We compared ileal Clostridium perfringens quantification results produced by real-time PCR and culture-based methods in broiler chickens in a challenge model of necrotic enteritis. Assessment of the relative standard deviations (RSDs) revealed that the real-time PCR assay generated a smaller standard deviation and thus was more precise than the culture-based method. Linear regression analysis indicated that the bacterial counts of these two methods were highly correlated (R-2 = 0.845). We suggest that real-time PCR could be a replacement of the culture method for quantifying C. perfringens in the intestinal tracts of broiler chickens.

引用

页码：1135 / 1139

页数：5

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