Expression of Recombinant Potato leafroll virus Structural and Non-structural Proteins for Antibody Production

被引：7
|
作者：
Plchova, Helena [1 ]
Moravec, Tomas [1 ]
Dedic, Petr [2 ]
Cerovska, Noemi [1 ]
机构：
[1] Acad Sci Czech Republ, Inst Expt Bot, VVI, Prague 16000 6, Czech Republic
[2] Potato Res Inst, Havlickuv Brod 58001, Czech Republic
来源：
JOURNAL OF PHYTOPATHOLOGY | 2011年 / 159卷 / 02期
关键词：
Potato leafroll virus; recombinant viral antigen; antibody production; MOP-TOP-VIRUS; POLYCLONAL ANTIBODIES; COAT PROTEIN; ESCHERICHIA-COLI;
D O I：
10.1111/j.1439-0434.2010.01740.x
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Vector pMPM-A4 Omega and vectors pQE-30 and pET-45b(+) containing the 6x His-tag sequence were used for expression of Potato leafroll virus (PLRV) structural and non-structural proteins in Escherichia coli. Coat protein (CP) and RNA-dependent RNA polymerase (RdRp)-fragments RdRp(43-616) and RdRp(304-537) were chosen for expression. A high level of CP and RdRp(304-537) was obtained only in an expression system using pET-45b(+) vector and E. coli Rosetta-gami 2(DE3) cells. After purification, the His-tagged PLRV proteins were used for immunization of rabbits.
引用
收藏
页码：130 / 132
页数：3
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