Measuring cell displacements in opaque tissues: dynamic light scattering in the multiple scattering regime

被引：3
|
作者：
Brunel, Benjamin [1 ]
Levy, Vincent [1 ]
Millet, Arnaud [2 ,3 ]
Dolega, Monika Elzbieta [1 ]
Delon, Antoine [1 ]
Pierrat, Romain [4 ]
Cappello, Giovanni [1 ]
机构：
[1] Univ Grenoble Alpes, Lab Interdisciplinaire Phys, CNRS, F-38000 Grenoble, France
[2] Univ Grenoble Alpes, CNRS, UMR 5309, Lnserm U1209,Inst Adv Biosci, F-38000 Grenoble, France
[3] Univ Hosp Grenoble Alpes, Res Dept, F-38000 Grenoble, France
[4] PSL Univ, CNRS, Inst Langevin, ESPCI Paris, 1 Rue Jussieu, F-75005 Paris, France
来源：
BIOMEDICAL OPTICS EXPRESS | 2020年 / 11卷 / 04期
关键词：
DIFFUSING-WAVE SPECTROSCOPY; LASER-DOPPLER MEASUREMENTS; OPTICAL-PHASE CONJUGATION; BLOOD-FLOW; TURBIDITY SUPPRESSION; SOLID STRESS; LIMIT; MICROSCOPY; SINGLE; GROWTH;
D O I：
10.1364/BOE.388360
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Coherent light scattered by tissues brings structural and dynamic information, at depth, that standard imaging techniques cannot reach. Dynamics of cells or sub-cellular elements can be measured thanks to dynamic light scattering in thin samples (single scattering regime) or thanks to diffusive wave spectroscopy in thick samples (diffusion regime). Here, we address the intermediate regime and provide an analytical relationship between scattered light fluctuations and the distribution of cell displacements as a function of time. We illustrate our method by characterizing cell motility inside half millimeter thick multicellular aggregates. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
引用
收藏
页码：2277 / 2297
页数：21
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