Comparison of nasopharyngeal aspirate with flocked swab for PCR-detection of respiratory viruses in children

被引：16

作者：

Tunsjo, Hege Smith ^{[1
]}

Berg, Are Stuitz ^{[2
]}

Inchley, Christopher Steven ^{[2
]}

Roberg, Irmelin Kittelsen ^{[1
]}

Leegaard, Truls Michael ^{[3
,4
]}

机构：

[1] Akershus Univ Hosp, Dept Multidisciplinary Lab Med & Biochem, Div Diagnost & Technol, Genet Unit, N-1478 Nordbyhagen, Norway

[2] Akershus Univ Hosp, Dept Child & Adolescent Med, N-1478 Nordbyhagen, Norway

[3] Akershus Univ Hosp, Dept Microbiol & Infect Control, Div Diagnost & Technol, Genet Unit, N-1478 Nordbyhagen, Norway

[4] Univ Oslo, Inst Clin Med, Oslo, Norway

来源：

APMIS | 2015年 / 123卷 / 06期

关键词：

Aspirate; flocked swab; respiratory viruses; PCR; REAL-TIME PCR; INFECTIONS;

D O I：

10.1111/apm.12375

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Fast- and high-throughput molecular workflows require sample matrices to be suitable for automation. Respiratory swabs are better suited for this purpose compared to the more viscous nasopharyngeal aspirates. Samples collected by nasopharyngeal aspiration and nasopharyngeal flocked swab from 81 children were compared for detection and recovery of respiratory viruses. Using real-time RT-PCR, no statistically significant differences in virus detection between the two sample types were found, supporting the use of flocked swabs in children aged one month to two years.

引用

页码：473 / 477

页数：5

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