Loop-mediated isothermal amplification for the rapid detection of Gardnerella vaginalis

被引：8
|
作者：
Higashide, Satoshi [1 ]
Cho, Otomi [1 ]
Matsuda, Yuko [1 ,2 ]
Ogishima, Daiki [2 ]
Kurakado, Sanae [1 ]
Sugita, Takashi [1 ]
机构：
[1] Meiji Pharmaceut Univ, Dept Microbiol, 2-522-1 Noshio, Tokyo 2048588, Japan
[2] Juntendo Nerima Hosp, Dept Obstet & Gynecol, 3-1-10 Takanodai, Tokyo 1778521, Japan
来源：
MICROBIOLOGY AND IMMUNOLOGY | 2018年 / 62卷 / 09期
关键词：
bacterial vaginosis; Gardnerella vaginalis; loop-mediated isothermal amplification; rapid detection; BACTERIAL VAGINOSIS; PRETERM DELIVERY; GRAM STAIN; DIAGNOSIS; ASSAY; LAMP; ALIGNMENT; ACCURATE; LOADS;
D O I：
10.1111/1348-0421.12641
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The aim of this study was to develop a method for the rapid detection of Gardnerella vaginalis, which is proposed to play a key role in the pathogenesis of bacterial vaginosis. Specific loop-mediated isothermal amplification (LAMP) primers were designed and used to detect target DNA within 45min under isothermal conditions. Comparative screening indicated that the LAMP assay is superior to PCR in terms of rapidity, and is equivalent in sensitivity and specificity. This LAMP assay can be used for rapid screening and detection of G. vaginalis in vaginal samples; the limit of detection is 10 fg DNA.
引用
收藏
页码：607 / 611
页数：5
相关论文
共 50 条
  • [31] Rapid and Sensitive Detection of Lophophora williamsii by Loop-Mediated Isothermal Amplification
    Sasaki, Yohei
    Fujimoto, Tsuguto
    Aragane, Masako
    Yasuda, Ichiro
    Nagumo, Seiji
    BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2009, 32 (05) : 887 - 891
  • [32] Establishment of loop-mediated isothermal amplification for rapid detection of Pseudomonas aeruginosa
    Li, Chao
    Shi, Yaoqiang
    Yang, Guangying
    Xia, Xue-Shan
    Mao, Xiaoqin
    Fang, Yue
    Zhang, A-Mei
    Song, Yuzhu
    EXPERIMENTAL AND THERAPEUTIC MEDICINE, 2019, 17 (01) : 131 - 136
  • [33] Sensitive and rapid detection of Zika virus by loop-mediated isothermal amplification
    Zhao, Jiangtao
    Feng, Ruo
    VIRUS GENES, 2019, 55 (01) : 43 - 50
  • [34] Loop-mediated isothermal amplification for rapid detection of Bacillus anthracis spores
    Yan-Mei Qiao
    Yong-Chao Guo
    Xian-En Zhang
    Ya-Feng Zhou
    Zhi-Ping Zhang
    Hong-Ping Wei
    Rui-Fu Yang
    Dian-Bing Wang
    Biotechnology Letters, 2007, 29 : 1939 - 1946
  • [35] Loop-mediated Isothermal Amplification Method for Rapid Detection of Shigella dysenteriae
    Zhong, Qingping
    Wang, Li
    Wang, Bin
    Chen, Hongyuan
    MEDICAL MATERIALS AND ENGINEERING, 2012, 140 : 369 - +
  • [36] Sensitive and rapid detection of Zika virus by loop-mediated isothermal amplification
    Jiangtao Zhao
    Ruo Feng
    Virus Genes, 2019, 55 : 43 - 50
  • [37] Loop-Mediated Isothermal Amplification Targeting Actin DNA of Trichomonas vaginalis
    Goo, Youn-Kyoung
    Shin, Won-Sik
    Yang, Hye-Won
    Joo, So-Young
    Song, Su-Min
    Ryu, Jae-Sook
    Kong, Hyun-Hee
    Lee, Won-Ki
    Chung, Dong-Il
    Hong, Yeonchul
    KOREAN JOURNAL OF PARASITOLOGY, 2016, 54 (03): : 329 - 334
  • [38] Rapid Detection of Group I Avian Adenoviruses by a Loop-Mediated Isothermal Amplification
    Xie, Zhixun
    Tang, Yi
    Fan, Qing
    Liu, Jiabo
    Pang, Yaoshan
    Deng, Xianwen
    Xie, Zhiqin
    Peng, Yi
    Xie, Liji
    Khan, Mazhar I.
    AVIAN DISEASES, 2011, 55 (04) : 575 - 579
  • [39] Rapid detection of ermB gene in Clostridium difficile by loop-mediated isothermal amplification
    Lin, Minyi
    Liu, Wei
    Wang, Pu
    Tan, Jiasheng
    Zhou, Youlian
    Wu, Peiqun
    Zhang, Ting
    Yuan, Jing
    Chen, Ye
    JOURNAL OF MEDICAL MICROBIOLOGY, 2015, 64 : 854 - 861
  • [40] Establishment of Multiplex Loop-Mediated Isothermal Amplification for Rapid Detection of Genitourinary Mycoplasma
    Wang, Yichao
    Cheng, You
    Xie, Jiaogui
    Liu, Yunde
    CLINICAL LABORATORY, 2018, 64 (7-8) : 1217 - 1224
12345