Protection against rotavirus infections by DNA vaccination

被引:79
|
作者
Herrmann, JE
Chen, SC
Fynan, EF
Santoro, JC
Greenberg, HB
Wang, SX
Robinson, HL
机构
[1] UNIV MASSACHUSETTS,SCH MED,DEPT PATHOL,WORCESTER,MA 01605
[2] WORCESTER STATE COLL,DEPT BIOL,WORCESTER,MA
[3] STANFORD UNIV,SCH MED,DIV GASTROENTEROL,STANFORD,CA
来源
关键词
D O I
10.1093/infdis/174.Supplement_1.S93
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
DNA vaccines encoding for murine rotavirus proteins VP4, VP6, or VP7 were tested in adult BALB/c mice for their ability to induce immune responses and protect against rotavirus challenge. A gene gun was used to inoculate vaccines into the epidermis, Rotavirus-specific serum antibodies, as measured by ELISA, and virus-specific cytotoxic T lymphocyte responses were generated by each of the three vaccines, but virus-neutralizing antibodies were detected only in mice that were inoculated with DNA vaccines encoding for VP4 and VP7. Efficacy of the vaccines was determined by challenge with 100 ID50 of homotypic rotavirus. Each of the three vaccines was effective in protecting mice against infection after rotavirus challenge as determined by reduction (P < .001) in virus excretion in mice receiving the DNA vaccines. These results demonstrate that DNA vaccination has potential as a new approach for control of rotavirus infections.
引用
收藏
页码:S93 / S97
页数:5
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