DNA Typing for the Identification of Old Skeletal Remains from Korean War Victims

被引：16

作者：

Lee, Hwan Young ^{[1
,2
,3
]}

Kim, Na Young ^{[1
,2
]}

Park, Myung Jin ^{[1
,2
]}

Sim, Jeong Eun ^{[1
,2
]}

Yang, Woo Ick ^{[1
,2
]}

Shin, Kyoung-Jin ^{[1
,2
,3
]}

机构：

[1] Yonsei Univ, Coll Med, Dept Forens Med, Seoul 120752, South Korea

[2] Yonsei Univ, Coll Med, Brain Korea Project Med Sci 21, Seoul 120752, South Korea

[3] Yonsei Univ, Human Identificat Res Ctr, Seoul 120752, South Korea

来源：

JOURNAL OF FORENSIC SCIENCES | 2010年 / 55卷 / 06期

关键词：

forensic science; DNA typing; skeletal remains; DNA extraction; mitochondrial DNA; autosomal short tandem repeat; Y-chromosomal short tandem repeat; mini-STR; CONTROL REGION SEQUENCES; MINI-PRIMER SET; POPULATION-DATA; STR LOCI; EXTRACTION; VALIDATION; MULTIPLEX; DATABASE; BONES;

D O I：

10.1111/j.1556-4029.2010.01411.x

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

The identification of missing casualties of the Korean War (1950-1953) has been performed using mitochondrial DNA (mtDNA) profiles, but recent advances in DNA extraction techniques and approaches using smaller amplicons have significantly increased the possibility of obtaining DNA profiles from highly degraded skeletal remains. Therefore, 21 skeletal remains of Korean War victims and 24 samples from biological relatives of the supposed victims were selected based on circumstantial evidence and/or mtDNA-matching results and were analyzed to confirm the alleged relationship. Cumulative likelihood ratios were obtained from autosomal short tandem repeat, Y-chromosomal STR, and mtDNA-genotyping results, and mainly confirmed the alleged relationship with values over 105. The present analysis emphasizes the value of mini- and Y-STR systems as well as an efficient DNA extraction method in DNA testing for the identification of old skeletal remains.

引用

页码：1422 / 1429

页数：8

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