The Influence of Copolymer Composition on PLGA/nHA Scaffolds' Cytotoxicity and In Vitro Degradation

被引：35
|
作者：
Diaz, Esperanza [1 ,2 ]
Puerto, Igor [1 ]
Ribeiro, Silvie [3 ]
Lanceros-Mendez, Senentxu [2 ,3 ,4 ]
Manuel Barandiarian, Jose [2 ]
机构：
[1] Univ Pais Vasco UPV EHU, Dept Ingn Minera Met & Ciencia Mat, Portugalete 48920, Spain
[2] BCMaterials, Parque Cient & Tecnol Bizkaia, Derio 48160, Spain
[3] Univ Minho, Ctr Dept Fis, P-4710057 Braga, Portugal
[4] Basque Fdn Sci, Ikerbasque, Bilbao 48013, Spain
来源：
NANOMATERIALS | 2017年 / 7卷 / 07期
关键词：
scaffolds; PLGA; nHA; cytotoxicity; in vitro degradation; HYDROLYTIC DEGRADATION; TISSUE; BIOMATERIALS;
D O I：
10.3390/nano7070173
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The influence of copolymer composition on Poly(Lactide-co-Glycolide)/nanohydroxyapatite (PLGA/nHA) composite scaffolds is studied in the context of bone tissue engineering and regenerative medicine. The composite scaffolds are fabricated by thermally-induced phase separation and the effect of bioactive nanoparticles on their in vitro degradation in phosphate-buffered solution at 37 degrees C is analyzed over eight weeks. The indirect cytotoxicity evaluation of the samples followed an adaptation of the ISO 10993-5 standard test method. Based on the measurement of their molecular weight, molar mass, pH, water absorption and dimensions, the porous scaffolds of PLGA with a lower lactide/glycolide (LA/GA) molar ratio degraded faster due to their higher hydrophilicity. All of the samples without and with HA are not cytotoxic, demonstrating their potential for tissue engineering applications.
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页数：16
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