Detection of Pathogens of Acute Febrile Illness Using Polymerase Chain Reaction from Dried Blood Spots

被引：1

作者：

Grundy, Brian ^{[1
]}

Panzner, Ursula ^{[2
,3
,4
]}

Liu, Jie ^{[1
]}

Jeon, Hyon Jin ^{[2
,5
]}

Im, Justin ^{[2
]}

von Kalckreuth, Vera ^{[2
]}

Konings, Frank ^{[2
]}

Pak, Gi Deok ^{[2
]}

Espinoza, Ligia Maria Cruz ^{[2
]}

Bassiahi, Abdramane Soura ^{[6
]}

Gasmelseed, Nagla ^{[7
]}

Rakotozandrindrainy, Raphae L. ^{[8
]}

Stroup, Suzanne ^{[1
]}

Houpt, Eric ^{[1
]}

Marks, Florian ^{[2
,5
,8
]}

机构：

[1] Univ Virginia, Div Infect Dis & Int Hlth, 345 Crispell Dr, Charlottesville, VA 22908 USA

[2] Int Vaccine Inst, Seoul, South Korea

[3] Swiss Trop & Publ Hlth Inst, Basel, Switzerland

[4] Univ Basel, Basel, Switzerland

[5] Univ Cambridge, Cambridge Inst Therapeut Immunol & Infect Dis, Cambridge Biomed Campus, Sch Clin Med, Cambridge, England

[6] Univ Ouagadougou, Inst Supeirieur Sci Populat, Ouagadougou, Burkina Faso

[7] Univ Gezira, Fac Med, Wad Madani, Sudan

[8] Univ Antananarivo, Antananarivo, Madagascar

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2022年 / 106卷 / 02期

基金：

美国国家卫生研究院; 比尔及梅琳达.盖茨基金会;

关键词：

HOLE-PUNCH; SURVEILLANCE; PCR; CONTAMINATION; DISEASE; AFRICA; SERUM; DNA;

D O I：

10.4269/ajtmh.21-0814

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Quantitative polymerase chain reaction (qPCR) of dried blood spots (DBS) for pathogen detection is a potentially convenient method for infectious disease diagnosis. This study tested 115 DBS samples paired with whole blood specimens of children and adolescent from Burkina Faso, Sudan, and Madagascar by qPCR for a wide range of pathogens, including protozoans, helminths, fungi, bacteria, and viruses. Plasmodium spp. was consistently detected from DBS but yielded a mean cycle threshold (Ct) 5.7 6 1.6 higher than that from whole blood samples. A DBS qPCR Ct cutoff of 27 yielded 94.1% sensitivity and 95.1% specificity against the whole blood qPCR cutoff of 21 that has been previously suggested for malaria diagnosis. For other pathogens investigated, DBS testing yielded a sensitivity of only 8.5% but a specificity of 98.6% compared with whole blood qPCR. In sum, direct PCR of DBS had reasonable performance for Plasmodium but requires further investigation for the other pathogens assessed in this study.

引用

页码：454 / 456

页数：3

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