Purification of a polyphenol oxidase isoform from potato (Solanum tuberosum) tubers

被引：54
|
作者：
Marri, C [1 ]
Frazzoli, A [1 ]
Hochkoeppler, A [1 ]
Poggi, V [1 ]
机构：
[1] Univ Bologna, Dept Ind Chem, I-40136 Bologna, Italy
来源：
PHYTOCHEMISTRY | 2003年 / 63卷 / 07期
关键词：
Solanum tuberosum; Solanaceae; potato tuber; enzyme expression; purification; polyphenol oxidase;
D O I：
10.1016/S0031-9422(03)00353-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A different expression pattern of polyphenol oxidases has been observed during storage in cultivars of potato (Solanum tuberosum L.) featuring different length of dormancy: a short-dormant cultivar showed, at the end of the dormancy, both the highest polyphenol oxidase activity and the largest number of enzyme isoforms. An isoform of polyphenol oxidase isolated at the end of the physiological dormancy from a short-dormant cultivar has been purified to homogeneity by means of column chromatography on phenyl Sepharose and on Superdex 200. The purification factor has been determined equal to 88, and the molecular mass of the purified isoform has been estimated to be 69 and 340 kDa by SIDS polyacrylamide get electrophoresis and gel filtration on Superdex 200, respectively, indicating this PPO isoform as a multimer. The corresponding zymogram features a diffused single band at the cathodic region of the gel and the pi of this polyphenol oxidase has been calculated equal to 6.5. (C) 2003 Elsevier Ltd. All rights reserved.
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页码：745 / 752
页数：8
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