Recurrent laryngeal nerve post-natal development in rats

Studies on the normal pattern of development and post-natal maturation of the recurrent laryngeal nerve in rats are scanty. The aim of the present study was to investigate the normal microscopic aspects of the recurrent laryngeal nerve myelinated fibers in the post-natal developing rat, with special attention to longitudinal morphology and lateral symmetry. Fifteen male rats were divided into experimental groups according to age 20, 55, 76, 150 and 250 days. A female group aged between 76 and 150 days was also used. Right and left RLN were studied by light microscopy at proximal, medium and distal segments and morphometric data comparisons were made between sides, segments, ages and gender. Our results showed that the left recurrent laryngeal nerves were significantly longer than the right in all ages studied and this difference increased with ageing. There was a slight decrease in the myelinated fiber number, according to proximal to distal gradient, but a significant decrease was observed only on older animals (ages 55 (both sides), 76 (left side), and 150 (left side) days). This difference was also observed for female rats (left side). No differences between ages were observed. There was an age-dependent difference on ranges and histograms form (younger animals showed a unimodal histogram while older animals showed a bimodal one), with no significant differences between segments or sides, in all groups studied. Also, no differences between males and females of the same age were observed. In conclusion, the RLN alters its morphology due to development and ageing and the present study describe normal patterns of the recurrent laryngeal nerve development in rats that can be useful for a better understanding of pathological alterations on experimental neuropathies involving the laryngeal nerves. (c) 2007 Elsevier B.V. All rights reserved.