NIR-II fluorescence in vivo functional bioimaging

Fluorescence bioimaging in the second near-infrared spectral region (NIR-II, 900-1700 nm) can provide advantages of high spatial resolution and large penetration depth, due to low light scattering. In addition, since long-wavelength light with low photon energy is utilized as the excitation and less absorbed by the biological tissues, NIR-II fluorescence bioimaging also has low autofluorescence, as well as negligible photodamage towards biosamples. During the past few years, NIR-II fluorescence bioimaging has experienced rapid development. In this paper, we will introduce some recent research works about NIR-II fluorescence bioimaging in our research group, such as video-rate whole-body/brain/tumour angiography, organ imaging, as well as diagnosis and imaging guided treatment of tumour, on mice and monkeys.