Identification of ABCC2 as a binding protein of Cry1Ac onbrush border membrane vesicles from Helicoverpa armigera by an improved pull-down assay

被引：35
|
作者：
Zhou, Zishan [1 ,2 ]
Wang, Zeyu [1 ]
Liu, Yuxiao [2 ]
Liang, Gemei [2 ]
Shu, Changlong [2 ]
Song, Fuping [2 ]
Zhou, Xueping [1 ,2 ]
Bravo, Alejandra [3 ]
Soberon, Mario [3 ]
Zhang, Jie [2 ]
机构：
[1] Zhejiang Univ, Inst Biotechnol, State Key Lab Rice Biol, 866 Yuhangtang Rd, Hangzhou 310012, Zhejiang, Peoples R China
[2] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China
[3] Univ Nacl Autonoma Mexico, Inst Biotecnol, Apdo Postal 510-3, Cuernavaca 62250, Morelos, Mexico
来源：
MICROBIOLOGYOPEN | 2016年 / 5卷 / 04期
基金：
中国国家自然科学基金; 中国博士后科学基金;
关键词：
ABCC2; alkaline phosphatase; aminopeptidase-N; Bacillus thuringiensis; cadherin; Cry1Ac; Helicoverpa armigera; BACILLUS-THURINGIENSIS CRY1A; ANCHORED ALKALINE-PHOSPHATASE; DELTA-ENDOTOXIN BINDING; MORI MIDGUT MEMBRANE; HELIOTHIS-VIRESCENS; AMINOPEPTIDASE-N; MANDUCA-SEXTA; BOMBYX-MORI; INSECTICIDAL ACTIVITY; PLUTELLA-XYLOSTELLA;
D O I：
10.1002/mbo3.360
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Cry1Ac toxin-binding proteins from Helicoverpa armigera brush border membrane vesicles were identified by an improved pull-down method that involves coupling Cry1Ac to CNBr agarose combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS). According to the LC-MS/MS results, Cry1Ac toxin could bind to six classes of aminopeptidase-N, alkaline phosphatase, cadherin-like protein, ATP-binding cassette transporter subfamily C protein (ABCC2), actin, ATPase, polycalin, and some other proteins not previously characterized as Cry toxin-binding molecules such as dipeptidyl peptidase or carboxyl/choline esterase and some serine proteases. This is the first report that suggests the direct binding of Cry1Ac toxin to ABCC2 in H.armigera.
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页码：659 / 669
页数：11
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