Hybridity Assessment in Experimental F1s of Rice (Oryza sativa L.) using Microsatellite Markers

The present investigation was undertaken to assess the hybridity of experimental F-1 plants derived from the crossing of salinity and/or submergence tolerant landraces with the high-yielding popular varieties of the west coast region of India. Seven microsatellite markers were utilized for confirming the hybridity of the 16 F-1 plants developed from four cross combinations, viz. Karjat-3 X KS-19-2 (Cross 1), Goa dhan-3 X Jaddubatta (Cross 2), Jaya X KS-19-2 (Cross 3) and Karjat-3 X Jaddubatta (Cross 4). Polymorphism survey using above seven markers between the parents of all the four crosses revealed that six of them were polymorphic, whereas one marker was monomorphic. The marker RM21539 was found to be polymorphic in all the four cross combinations, followed by RM493, RM3412, RM206 and RM10843 exhibiting polymorphism in three crosses, while marker RM25181 was polymorphic in two crosses. The screening of F(1)s with polymorphic markers revealed that the F(1)s from Cross 3 exhibited heterozygosity (showing alleles from both the parents) at six marker loci, whereas the F(1)s from Cross 1 and Cross 4 at five loci and Cross 2 at two marker loci, thereby confirming hybridity in all the 16 tested F-1 plants.