α-Galactosidases production by Debaryomyces hansenii UFV-1

被引：7
|
作者：
Viana, Pollyanna Amaral [1 ,2 ]
de Rezende, Sebastiao Tavares [1 ]
Lopes Passos, Flavia Maria [1 ]
Machado, Solimar Goncalves [1 ]
Maitan, Gabriela Picollo [1 ]
da Silva Coelho, Vinicio Tadeu [3 ]
Guimaraes, Valeria Monteze [1 ]
机构：
[1] Univ Fed Vicosa, BIOAGRO, BR-36570000 Vicosa, MG, Brazil
[2] Univ Fed Vicosa, BR-35690000 Florestal, MG, Brazil
[3] Univ Fed Minas Gerais, Dept Bioquim & Imunol ICB, BR-31270901 Belo Horizonte, MG, Brazil
来源：
FOOD SCIENCE AND BIOTECHNOLOGY | 2011年 / 20卷 / 03期
关键词：
Debaryomyces hansenii UFV-1; alpha-galactosidase; culture media; carbon source; enzyme production; HYDROLYSIS; OLIGOSACCHARIDES; TRANSPORTER; PROTEINS; WOOD;
D O I：
10.1007/s10068-011-0085-7
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Extracellular and intracellular alpha-galactosidases were produced by yeast Debaryomyces hansenii UFV-1 grown on different media with several carbon sources. D. hansenii grown in YP-medium (1% yeast extract and 2% peptone) presented maximum cell mass (8.45 mg/mL) after 36 h of cultivation, with lactose as carbon source, followed by sucrose, glucose, raffinose, and galactose. Higher extracellular and intracellular alpha-galactosidases activities were observed at 48 h of D. hansenii cultivation in YPmedium containing galactose (0.97 and 5.27 U/mL) and lactose (1.28 and 4.88 U/mL), supporting the evidence for the model of induction for the yeast GAL/MEL regulon, such as described in Sacharomyces cereviseae.
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页码：601 / 606
页数：6
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