Circadian Entrainment of Drosophila Melanogaster

被引：2

作者：

Dada, Austin O. ^{[1
]}

Nguyen, Minh Q. ^{[1
]}

Peterson, Shea M. ^{[1
]}

Ngo, Vy T. ^{[1
]}

Cornelio-Parra, Dayanne, V ^{[1
]}

Omer, Bwaar S. ^{[1
]}

Thapa, Ada ^{[2
]}

Rapp, Sarah R. ^{[1
]}

Cloud, Veronica J. ^{[1
]}

Mohan, Ryan D. ^{[1
]}

机构：

[1] Univ Missouri Kansas City, Sch Biol & Chem Sci, Kansas City, MO 64110 USA

[2] Univ Maryland, Sch Publ Hlth, College Pk, MD 20742 USA

来源：

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2020年 / 160期

关键词：

Biology; Issue; 160; Drosophila; circadian entrainment; immunofluorescence; protein extraction; fixation; neurobiology; PERIOD GENE-PRODUCT; CLOCK; PROTEIN; RHYTHMS; LIGHT; TIME;

D O I：

10.3791/61176

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Nearly universal among organisms, circadian rhythms coordinate biological activity to earth's orbit around the sun. To identify factors creating this rhythm and to understand the resulting outputs, entrainment of model organisms to defined circadian time-points is required. Here we detail a procedure to entrain many Drosophila to a defined circadian rhythm. Furthermore, we detail post-entrainment steps to prepare samples for immunofluorescence, nucleic acid, or protein extraction-based analysis.

引用

页码：1 / 4

页数：4

共 50 条

[21] Circadian dysfunction reduces lifespan in Drosophila melanogaster
Kumar, S
Mohan, A
Sharma, VK
CHRONOBIOLOGY INTERNATIONAL, 2005, 22 (04) : 641 - 653
[22] The neuroarchitecture of the circadian clock in the brain of Drosophila melanogaster
Helfrich-Förster, C
MICROSCOPY RESEARCH AND TECHNIQUE, 2003, 62 (02) : 94 - 102
[23] Circadian rhythms in olfactory responses of Drosophila melanogaster
Krishnan, B
Dryer, SE
Hardin, PE
NATURE, 1999, 400 (6742) : 375 - 378
[24] Circadian rhythms in olfactory responses of Drosophila melanogaster
Balaji Krishnan
Stuart E. Dryer
Paul E. Hardin
Nature, 1999, 400 : 375 - 378
[25] Re-entrainment of Drosophila circadian rhythm by red light
Hanai, Shuji
Hanasaka, Yasutaka
Ishida, Norio
NEUROSCIENCE RESEARCH, 2008, 61 : S63 - S63
[26] Cryptochrome-Dependent and -Independent Circadian Entrainment Circuits in Drosophila
Yoshii, Taishi
Hermann-Luibl, Christiane
Kistenpfennig, Christa
Schmid, Benjamin
Tomioka, Kenji
Helfrich-Foerster, Charlotte
JOURNAL OF NEUROSCIENCE, 2015, 35 (15): : 6131 - 6141
[27] Synergic Entrainment of Drosophila's Circadian Clock by Light and Temperature
Yoshii, Taishi
Vanin, Stefano
Costa, Rodolfo
Helfrich-Foerster, Charlotte
JOURNAL OF BIOLOGICAL RHYTHMS, 2009, 24 (06) : 452 - 464
[28] Circadian regulation of response to oxidative stress in Drosophila melanogaster
Krishnan, Natraj
Davis, Andrew J.
Giebultowicz, Jadwiga M.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2008, 374 (02) : 299 - 303
[29] Circadian Regulation of Glutathione Levels and Biosynthesis in Drosophila melanogaster
Beaver, Laura M.
Klichko, Vladimir I.
Chow, Eileen S.
Kotwica-Rolinska, Joanna
Williamson, Marisa
Orr, William C.
Radyuk, Svetlana N.
Giebultowicz, Jadwiga M.
PLOS ONE, 2012, 7 (11):
[30] Neural circuits underlying circadian behavior in Drosophila melanogaster
Chang, DC
BEHAVIOURAL PROCESSES, 2006, 71 (2-3) : 211 - 225

← 1 2 3 4 5 →