Circadian Entrainment of Drosophila Melanogaster

被引：2

作者：

Dada, Austin O. ^{[1
]}

Nguyen, Minh Q. ^{[1
]}

Peterson, Shea M. ^{[1
]}

Ngo, Vy T. ^{[1
]}

Cornelio-Parra, Dayanne, V ^{[1
]}

Omer, Bwaar S. ^{[1
]}

Thapa, Ada ^{[2
]}

Rapp, Sarah R. ^{[1
]}

Cloud, Veronica J. ^{[1
]}

Mohan, Ryan D. ^{[1
]}

机构：

[1] Univ Missouri Kansas City, Sch Biol & Chem Sci, Kansas City, MO 64110 USA

[2] Univ Maryland, Sch Publ Hlth, College Pk, MD 20742 USA

来源：

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2020年 / 160期

关键词：

Biology; Issue; 160; Drosophila; circadian entrainment; immunofluorescence; protein extraction; fixation; neurobiology; PERIOD GENE-PRODUCT; CLOCK; PROTEIN; RHYTHMS; LIGHT; TIME;

D O I：

10.3791/61176

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Nearly universal among organisms, circadian rhythms coordinate biological activity to earth's orbit around the sun. To identify factors creating this rhythm and to understand the resulting outputs, entrainment of model organisms to defined circadian time-points is required. Here we detail a procedure to entrain many Drosophila to a defined circadian rhythm. Furthermore, we detail post-entrainment steps to prepare samples for immunofluorescence, nucleic acid, or protein extraction-based analysis.

引用

页码：1 / 4

页数：4

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