A semi-quantitative RT-PCR to assess differential expression levels of TCF3 gene in two Chinese indigenous chicken breeds

被引：0
|
作者：
Jin-Yu, Wang [1 ]
Liang-Yong, Bian [1 ]
Yan, Yang [1 ]
Lin-Chuan, Li [1 ]
Musa, H. Hassan [1 ]
Mekki, M. Dafalla [1 ,2 ]
机构：
[1] Yangzhou Univ, Coll Anim Sci & Technol, Dept Anim Genet Breeding & Reprod, Jiangsu, Peoples R China
[2] Univ Kordofan, Fac Nat Resources & Environm Studies, Dept Anim Sci, Kordofan, Sudan
来源：
JOURNAL OF ANIMAL AND VETERINARY ADVANCES | 2007年 / 6卷 / 02期
关键词：
TCF3; semi-quantitative RT-PCR; gene expression; chicken breeds; jinghai breed; sugin bread;
D O I：
暂无
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Semi-Quantitative Polymerase Chain Reaction (RT-PCR) was applied to quantify and compare variations in avian TCF3 expression level between two Chinese indigenous chicken breeds for different tissues. Six primer combinations in addition to beta-actin as an internal control were used. The expression level of TCF3 was greater in Jinghai breed than Suqin breed for different tissues. The variability of gene expression between two breeds showed 18.55, 37.85 and 66.15% higher expression in Jinghai breed than Suqin breed for kidney, lung and spleen tissues, respectively. Differences were found to be significant (p<0.05) only for expression level in lung and spleen tissues. Significant effect of sex upon TCF3 expression was detected for both breeds.
引用
收藏
页码：185 / 189
页数：5
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