Evaluation of semi-automatic 3D reconstruction for studying geometry of dendritic spines

被引:8
|
作者
Al-Absi, Abdel-Rahman [1 ,2 ]
Christensen, Heidi Sogaard [2 ,3 ]
Sanchez, Connie [4 ,5 ]
Nyengaard, Jens Randel [1 ,2 ]
机构
[1] Aarhus Univ, Core Ctr Mol Morphol, Sect Stereol & Microscopy, Dept Clin Med, Aarhus, Denmark
[2] Aarhus Univ, Ctr Stochast Geometry & Adv Bioimaging, Aarhus, Denmark
[3] Aalborg Univ, Dept Math Sci, Aalborg, Denmark
[4] Alkermes, Waltham, MA USA
[5] Aarhus Univ, Dept Clin Med, Translat Neuropsychiat Unit, Aarhus, Denmark
关键词
Golgi staining; Semi-automatic method; Spine reconstruction; TOOL;
D O I
10.1016/j.jchemneu.2018.10.008
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background: Spine geometry is considered to reflect synapse function. An accurate and fast method for 3D reconstruction of spines is considered a valuable tool for the purpose of studying spine geometry. Currently, most studies employ manual or automatic reconstruction methods, which still suffer from either poor accuracy or extreme time-consumption. The semi-automatic reconstruction method has previously been described as a time-economic and accurate tool for spine number counting. The purpose of this study is to further validate the semi-automatic method with regards to spine geometry investigation, by comparing it with the manual method as well as with the automatic method. Methods: In this study, dendritic trees of six pyramidal neurons that belong to layers II/III of mouse frontal cortex are stained using the Golgi method. Thereafter, spines from 42 dendritic branches are 3D reconstructed by manual, semi-automatic and automatic methods using Imaris software. Spine features, including spine volume, spine area, spine length and spine neck length, and the relative distribution of classified stubby, mushroom and thin spines are compared between the semi-automatic method and the two other methods. Results: Results from the semi-automatic and the manual reconstruction methods are in line with respect to all measured spine geometric features as well as spine classes. However, significant difference has been detected between the two methods and the automatic method in spine length, spine neck length and spine volume. Compared to the manual method, both the semi-automatic and the automatic methods have significantly reduced the spine reconstruction time. Conclusion: These findings suggest that the semi-automatic method may represent both a time-economic and reliable option for the purpose of studying spine geometry.
引用
收藏
页码:119 / 124
页数:6
相关论文
共 50 条
  • [1] A semi-automatic 3D reconstruction algorithm for telepresence
    Sarkis, Michel
    Diepold, Klaus
    [J]. ADVANCES IN VISUAL COMPUTING, PT 2, 2006, 4292 : 596 - +
  • [2] Semi-automatic 3D reconstruction of urban areas using epipolar geometry and template matching
    Dias, Jose Miguel Sales
    Bastos, Rafael
    Correia, Joao
    Vicente, Rodrigo
    [J]. COMPUTER-AIDED CIVIL AND INFRASTRUCTURE ENGINEERING, 2006, 21 (07) : 466 - 485
  • [3] Semi-automatic 3D reconstruction of middle and inner ear structures using CBCT
    Beguet, Florian
    Cresson, Thierry
    Schmittbuhl, Mathieu
    Doucet, Cedric
    Camirand, David
    Harris, Philippe
    Mari, Jean-Luc
    de Guise, Jacques
    [J]. COMPUTER METHODS IN BIOMECHANICS AND BIOMEDICAL ENGINEERING-IMAGING AND VISUALIZATION, 2023, 11 (05): : 2006 - 2019
  • [4] Evaluation of a semi-automatic segmentation algorithm in 3D intraoperative ultrasound brain angiography
    Chalopin, Claire
    Krissian, Karl
    Meixensberger, Juergen
    Muens, Andrea
    Arlt, Felix
    Lindner, Dirk
    [J]. BIOMEDICAL ENGINEERING-BIOMEDIZINISCHE TECHNIK, 2013, 58 (03): : 293 - 302
  • [5] Accuracy evaluation of the semi-automatic 3D modeling for historical building information models
    Anton, Daniel
    Medjdoub, Benachir
    Shrahily, Raid
    Moyano, Juan
    [J]. INTERNATIONAL JOURNAL OF ARCHITECTURAL HERITAGE, 2018, 12 (05) : 790 - 805
  • [6] 3D semi-automatic segmentation of the cochlea and inner ear
    Diao Xianfen
    Chen Siping
    Liang Changhong
    Wang Yuanmei
    [J]. 2005 27TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, VOLS 1-7, 2005, : 6285 - 6288
  • [7] Semi-automatic detection of faults in 3D seismic data
    Tingdahl, KM
    de Rooij, M
    [J]. GEOPHYSICAL PROSPECTING, 2005, 53 (04) : 533 - 542
  • [8] APPROACH FOR THE SEMI-AUTOMATIC VERIFICATION OF 3D BUILDING MODELS
    Helmholz, P.
    Belton, D.
    Moncrieff, S.
    [J]. ISPRS HANNOVER WORKSHOP 2013, 2013, 40-1 (W-1): : 121 - 126
  • [9] Development and evaluation of a semi-automatic 3D segmentation technique of the carotid arteries from 3D ultrasound images
    Gill, JD
    Ladak, HM
    Steinman, DA
    Fenster, A
    [J]. MEDICAL IMAGING 1999: IMAGE PROCESSING, PTS 1 AND 2, 1999, 3661 : 214 - 221
  • [10] A semi-automatic 3D ultrasound reconstruction method to assess the true severity of adolescent idiopathic scoliosis
    Quang N. Vo
    Lawrence H. Le
    Edmond Lou
    [J]. Medical & Biological Engineering & Computing, 2019, 57 : 2115 - 2128