Influence of mixotrophic growth on rhythmic oscillations in expression of metabolic pathways in diazotrophic cyanobacterium Cyanothece sp ATCC 51142

被引：4
|
作者：
Krishnakumar, S. [1 ]
Gaudana, Sandeep B. [1 ]
Digmurti, Madhuri G. [1 ]
Viswanathan, Ganesh A. [1 ]
Chetty, Madhu [2 ]
Wangikar, Pramod P. [1 ,3 ,4 ]
机构：
[1] Indian Inst Technol, Dept Chem Engn, Bombay 400076, Maharashtra, India
[2] Federat Univ Australia, Sch Informat Technol, Melbourne, Vic 3841, Australia
[3] Indian Inst Technol, Wadhwani Res Ctr Bioengn, Bombay 400076, Maharashtra, India
[4] Indian Inst Technol, DBT Pan IIT Ctr Bioenergy, Bombay 400076, Maharashtra, India
来源：
BIORESOURCE TECHNOLOGY | 2015年 / 188卷
关键词：
Transcriptomic analysis; Nitrogen fixation; Hydrogen production; Metabolic oscillations; Cyanobacteria; NITROGEN-FIXING CYANOBACTERIA; SP STRAIN ATCC-51142; CONTINUOUS-LIGHT; CONCENTRATING MECHANISM; HYDROGEN-PRODUCTION; GENUS CYANOTHECE; SYNECHOCYSTIS; FIXATION; CARBON; IDENTIFICATION;
D O I：
10.1016/j.biortech.2015.02.016
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
This study investigates the influence of mixotrophy on physiology and metabolism by analysis of global gene expression in unicellular diazotrophic cyanobacterium Cyanothece sp. ATCC 51142 ( henceforth Cyanothece 51142). It was found that Cyanothece 51142 continues to oscillate between photosynthesis and respiration in continuous light under mixotrophy with cycle time of similar to 13 h. Mixotrophy is marked by an extended respiratory phase compared with photoautotrophy. It can be argued that glycerol provides supplementary energy for nitrogen fixation, which is derived primarily from the glycogen reserves during photoautotrophy. The genes of NDH complex, cytochrome c oxidase and ATP synthase are significantly overexpressed in mixotrophy during the day compared to autotrophy with synchronous expression of the bidirectional hydrogenase genes possibly to maintain redox balance. However, nitrogenase complex remains exclusive to nighttime metabolism concomitantly with uptake hydrogenase. This study throws light on interrelations between metabolic pathways with implications in design of hydrogen producer strains. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：145 / 152
页数：8
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