Electrophysiological evidence for glial-subtype glutamate transporter functional expression in rat cerebellar granule neurons

被引：3

作者：

Mafra, RA

Leao, RM

Beirao, PSI

Cruz, JS

机构：

[1] Univ Fed Minas Gerais, ICB, Dept Bioquim & Imunol, Lab Membranas Excitaveis, BR-30161970 Belo Horizonte, MG, Brazil

[2] Univ Fed Paraiba, Ctr Ciencias Exatas & Nat, Dept Biol Mol, BR-58059900 Joao Pessoa, Paraiba, Brazil

[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Fisiol, BR-14049 Ribeirao Preto, SP, Brazil

来源：

BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH | 2003年 / 36卷 / 07期

关键词：

cerebellar granule neurons; excitatory amino-acid transporter type 2; patch clamp; glutamate; whole-cell voltage-clamp;

D O I：

10.1590/S0100-879X2003000700018

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A glutamate-sensitive inward current (Iglu) is described in rat cerebellar granule neurons and related to a glutamate transport mechanism. We examined the features of Iglu using the patch-clamp technique. In steady-state conditions the Iglu measured 8.14 +/- 1.9 pA. Iglu was identified as a voltage-dependent inward current showing a strong rectification at positive potentials. L-Glutamate activated the inward current in a dose-dependent manner, with a half-maximal effect at about 18 muM and a maximum increase of 51.2 +/- 4.4%. The inward current was blocked by the presence of dihydrokainate (0.5 mM), shown by others to readily block the GLT1 isoform. We thus speculate that Iglu could be attributed to the presence of a native glutamate transporter in cerebellar granule neurons.

引用

页码：951 / 957

页数：7

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