Facile synthesis of oligonucleotides on solid support using pyridine derivatives for amino and phosphate protection

被引：0

作者：

Sinha, S ^{[1
]}

Singh, RK ^{[1
]}

机构：

[1] Univ Allahabad, Dept Chem, Nucleic Acids Res Lab, Allahabad 211002, Uttar Pradesh, India

来源：

INDIAN JOURNAL OF CHEMISTRY SECTION B-ORGANIC CHEMISTRY INCLUDING MEDICINAL CHEMISTRY | 2003年 / 42卷 / 07期

关键词：

D O I：

暂无

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The picolinoyl, group has been used for amino protection in the case of all the three deoxynucleosides-dC, dA and dG, and good yields of N-protected nucleosides are obtained. This pyridine derivative along with another pyridine derivative, viz. (alpha-pyridyl) methyl, an autocatalytic phosphate protecting group, has been used successfully in the synthesis of two oligonucleotides, d(TACGGTTTGCT) and d(ACCGATATCGT). following solid phase methodology. The good yields of these 11-mers (48 and 45%, respectively) are attributed to the greater solubilising effect generated due to the combination of these two groups. The structures of these oligomers have been confirmed by enzymatic hydrolysis with snake venom phosphodiesterase followed by alkaline phosphatase.

引用

页码：1696 / 1700

页数：5

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