Peritoneal fluid from endometriosis patients switches differentiation of monocytes from dendritic cells to macrophages
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作者:
Na, Yong-Jin
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Pusan Natl Univ, Med Res Inst, Dept Obstet & Gynecol, Pusan 602739, South KoreaPusan Natl Univ, Med Res Inst, Dept Obstet & Gynecol, Pusan 602739, South Korea
Na, Yong-Jin
[1
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Jin, Jun-O
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Dong A Univ, Sch Med, Med Res Ctr Canc Mol Therapy, Pusan 602714, South Korea
Dong A Univ, Sch Med, Dept Biochem, Pusan 602714, South KoreaPusan Natl Univ, Med Res Inst, Dept Obstet & Gynecol, Pusan 602739, South Korea
Jin, Jun-O
[2
,3
]
Lee, Mi-Sook
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Dong A Univ, Sch Med, Med Res Ctr Canc Mol Therapy, Pusan 602714, South Korea
Dong A Univ, Sch Med, Dept Biochem, Pusan 602714, South KoreaPusan Natl Univ, Med Res Inst, Dept Obstet & Gynecol, Pusan 602739, South Korea
Lee, Mi-Sook
[2
,3
]
Song, Min-Gyu
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Dong A Univ, Sch Med, Med Res Ctr Canc Mol Therapy, Pusan 602714, South Korea
Dong A Univ, Sch Med, Dept Biochem, Pusan 602714, South KoreaPusan Natl Univ, Med Res Inst, Dept Obstet & Gynecol, Pusan 602739, South Korea
Song, Min-Gyu
[2
,3
]
Lee, Kyu-Sup
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机构:
Pusan Natl Univ, Med Res Inst, Dept Obstet & Gynecol, Pusan 602739, South KoreaPusan Natl Univ, Med Res Inst, Dept Obstet & Gynecol, Pusan 602739, South Korea
Lee, Kyu-Sup
[1
]
Kwak, Jong-Young
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Dong A Univ, Sch Med, Med Res Ctr Canc Mol Therapy, Pusan 602714, South Korea
Dong A Univ, Sch Med, Dept Biochem, Pusan 602714, South KoreaPusan Natl Univ, Med Res Inst, Dept Obstet & Gynecol, Pusan 602739, South Korea
Kwak, Jong-Young
[2
,3
]
机构:
[1] Pusan Natl Univ, Med Res Inst, Dept Obstet & Gynecol, Pusan 602739, South Korea
[2] Dong A Univ, Sch Med, Med Res Ctr Canc Mol Therapy, Pusan 602714, South Korea
[3] Dong A Univ, Sch Med, Dept Biochem, Pusan 602714, South Korea
Immunological abnormalities of cell-mediated and humoral immunity might be associated with the pathogenesis of endometriosis. This study has examined the effects of peritoneal fluid obtained from patients with endometriosis (ePF) on the phenotypic characteristics of macrophages and dendritic cells (DCs) derived from monocytes. Monocytes were obtained from healthy young volunteers and cultured with ePF (n = 12) or a control PF (cPF) (n = 5) in the presence or absence of macrophage-colony stimulating factor (M-CSF) or IL-4 plus granulocyte macrophage-colony stimulating factor (GM-CSF). The OF was demonstrated to increase expression levels of CD14 and CD64 on isolated monocytes in the presence or absence of M-CSF Compared with OF, addition of 10% ePF to GM-CSF plus IL-4-treated monocytes significantly down-regulated CD1a expression and up-regulated CD64 expression, but did not enhance expression levels of class II MHC. ePF had no effect, however, on tumor necrosis factor-a-induced maturation of DC. Levels of IL-6, IL-10 and M-CSF production were higher in ePF-treated than cPF-treated monocytes for both cell culture conditions with GM-CSF plus IL-4 and M-CSF. A neutralizing IL-6 antibody, but not an IL-10 antibody, abrogated the ePF-induced down-regulation of CD1a, up-regulation of CD64 and secretion of M-CSF. These results suggest that ePF favorably induces monocyte differentiation toward macrophages rather than DCs, and that this effect is mediated by IL-6. A reciprocal mode of cell differentiation between macrophages and DCs in response to ePF may be related to the pathogenesis of endometriosis. (c) 2007 Elsevier Ireland Ltd. All rights reserved.
机构:
Kings Coll London, Ctr Mol & Cellular Biol Inflammat, Div Immunol Infect & Inflammatory Dis, London SE1 1UL, EnglandKings Coll London, Ctr Mol & Cellular Biol Inflammat, Div Immunol Infect & Inflammatory Dis, London SE1 1UL, England
Geissmann, Frederic
Manz, Markus G.
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Univ Zurich Hosp, Dept Internal Med, Div Hematol, CH-8091 Zurich, SwitzerlandKings Coll London, Ctr Mol & Cellular Biol Inflammat, Div Immunol Infect & Inflammatory Dis, London SE1 1UL, England
Manz, Markus G.
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机构:
Jung, Steffen
Sieweke, Michael H.
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Univ Aix Marseille, CIML, F-13288 Marseille 09, France
INSERM, U631, F-13258 Marseille, France
CNRS, UMR6102, Marseille, FranceKings Coll London, Ctr Mol & Cellular Biol Inflammat, Div Immunol Infect & Inflammatory Dis, London SE1 1UL, England
Sieweke, Michael H.
Merad, Miriam
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机构:
Mt Sinai Sch Med, Icahn Med Inst, Inst Immunol, New York, NY 10029 USA
Mt Sinai Sch Med, Dept Gene & Cell Med, New York, NY 10029 USAKings Coll London, Ctr Mol & Cellular Biol Inflammat, Div Immunol Infect & Inflammatory Dis, London SE1 1UL, England
Merad, Miriam
Ley, Klaus
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机构:
La Jolla Inst Allergy & Immunol, Div Inflammat Biol, La Jolla, CA 92037 USAKings Coll London, Ctr Mol & Cellular Biol Inflammat, Div Immunol Infect & Inflammatory Dis, London SE1 1UL, England