HPLC-UV DETERMINATION OF MORPHINE IN HUMAN PLASMA AND ITS APPLICATION TO THE CLINICAL STUDY

被引：0
|
作者：
Szkutnik-Fiedler, Danuta [1 ]
Grzeskowiak, Edmund [1 ]
Gaca, Michal [2 ,3 ]
Borowicz, Magdalena [1 ]
机构：
[1] Poznan Univ Med Sci, Dept Clin Pharm & Biopharm, PL-61861 Poznan, Poland
[2] Poznan Univ Med Sci, Clin Obstet, PL-60535 Poznan, Poland
[3] Poznan Univ Med Sci, Clin Gynecol Anaesthesiol, PL-60535 Poznan, Poland
来源：
ACTA POLONIAE PHARMACEUTICA | 2011年 / 68卷 / 04期
关键词：
high performance liquid chromatography; UV detector; plasma samples; morphine; hysterectomy; pain; TANDEM MASS-SPECTROMETRY; PERFORMANCE LIQUID-CHROMATOGRAPHY; FLUORESCENCE DETECTION; GC-MS; LC-MS; BLOOD; URINE; 6-ACETYLMORPHINE; METABOLITES; CODEINE;
D O I：
暂无
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
A sensitive and specific high-performance liquid chromatography method with ultraviolet detection (HPLC-UV) has been developed for the quantification of morphine sulfate [(5 alpha,6 alpha)-7,8-didehydro-4,5-epoxy-17-methylmorphinan-3,6-dioll, (CAS: 52-26-6) in human plasma. The analyte was extracted from plasma samples with chloroform isopropyl alcohol (90:10, v/v) and analyzed on a Bondapak C-18 column. The calibration curves were linear within the range of 10-150 ng/mL. The lower limit of quantitation was 10 ng/mL with 0.5 mL plasma sample. The mean recovery of the drug from plasma samples was 83.39%. The results from analysis of quality-control samples at concentrations of 30, 75, and 150 ng/mL were indicative of good accuracy and precision. This method was successfully used to analyze morphine in plasma samples of patients after abdominal hysterectomy.
引用
收藏
页码：473 / 479
页数：7
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