Study on the role of environmental parameters and HIF-1A gene polymorphism in coronary collateral formation among patients with ischemic heart disease

Objectives: To evaluate the association between collateral formation and some environmental factors along with a polymorphism in HIF-1A gene in selected Iranian patients with CAD. Design and methods: Patients with >= 70% narrowing in at least one coronary vessel according to coronary angiography were enrolled. The patients' demographic, clinical and biochemical data were collected. The presence of C1772T polymorphisms within HIF-1A was analyzed using the polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP). Results: There is no significant difference between the patients with and without collaterals according to the frequency of T allele or HIF-1A variants. The higher severity of coronary vessel obstruction was positive predictor of collateral formation (OR = 1.026, 95%, CI: 1.02-0.04, p<0.001), whereas aging and cigarette smoking were negative predictors (OR = 0.95, 95% Cl: 0.91-0.99, p<0.05; OR = 0.30, 95% CI: 0.11-0.79, p <0.05; respectively). Conclusions: The findings indicate not any significant association between collateral formation and polymorphic variants of HIF-1A and P582S substitution does not appear to influence the collateral formation in patients with myocardial ischemia (C) 2011 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.