Development of quantitative PCR primers and probes for environmental DNA detection of amphibians in Ontario

被引：9

作者：

Beauclerc, Kaela ^{[1
]}

Wozney, Kristyne ^{[2
]}

Smith, Caleigh ^{[2
]}

Wilson, Chris ^{[2
]}

机构：

[1] Trent Univ, Wildlife Res & Monitoring Sect, Ontario Minist Nat Resources & Forestry, 2140 East Bank Dr, Peterborough, ON K9L 0G2, Canada

[2] Trent Univ, Aquat Res & Monitoring Sect, Ontario Minist Nat Resources & Forestry, 2140 East Bank Dr, Peterborough, ON K9L 0G2, Canada

来源：

CONSERVATION GENETICS RESOURCES | 2019年 / 11卷 / 01期

关键词：

Amphibian; eDNA; Quantitative PCR; GREAT CRESTED NEWT; EDNA;

D O I：

10.1007/s12686-017-0962-3

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

DNA from environmental samples (eDNA) is increasingly being used to detect and monitor elusive species. We developed species-specific eDNA primers and probes for qPCR detection of 24 amphibian species native to Ontario, Canada. Cross-species testing confirmed their high specificity and low cross-species amplification, as well as their ability to detect DNA from target species at low concentrations. These detection tools should prove useful for monitoring at-risk amphibian species throughout their native ranges.

引用

页码：43 / 46

页数：4

共 50 条

[31] Design and use of group-specific primers and probes for real-time quantitative PCR
Juntaek Lim
Seung Gu Shin
Seungyong Lee
Seokhwan Hwang
Frontiers of Environmental Science & Engineering in China, 2011, 5 : 28 - 39
[32] Development of specific PCR primers for identification and detection of Rhizopycnis vagum
Ghignone, S
Tamietti, G
Girlanda, M
EUROPEAN JOURNAL OF PLANT PATHOLOGY, 2003, 109 (08) : 861 - 870
[33] Development of Specific PCR Primers for Identification and Detection of Rhizopycnis vagum
Stefano Ghignone
Giacomo Tamietti
Mariangela Girlanda
European Journal of Plant Pathology, 2003, 109 : 861 - 870
[34] Development of PCR Primers and a DNA Macroarray for the Simultaneous Detection of Major Staphylococcus Species Using groESL Gene
Chiang, Yu-Cheng
Lu, Hsi-Chi
Li, Sheng-Chih
Chang, Yu-Hsin
Chen, Hsin-Yen
Lin, Chia-Wei
Tsen, Hau-Yang
FOODBORNE PATHOGENS AND DISEASE, 2012, 9 (03) : 249 - 257
[35] Development of PCR Primers for the Detection of Porcine DNA in Feed Using mtATP6 as the Target Sequence
Yoshida, Tomotaro
Nomura, Tetsuya
Shinoda, Naoki
Kusama, Toyoko
Kadowaki, Koh-ichi
Sugiura, Katsuaki
JOURNAL OF THE FOOD HYGIENIC SOCIETY OF JAPAN, 2009, 50 (02): : 89 - 92
[36] Development of Strain-Specific PCR Primers for Quantitative Detection of Bacillus mesentericus Strain TO-A in Human Feces
Sato, Naoki
Seo, Genichiro
Benno, Yoshimi
BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2014, 37 (01) : 123 - 129
[37] Development and Use of Quantitative Competitive PCR Assay for Detection of Poultry DNA in Sausage
Aslaminejad, Ali Asghar
Nassiry, Mohammad Reza
Farajollahi, Hadi
Mahdavi, Morteza
Sekhavati, Mohammad Hadi
Javadmanesh, Ali
FOOD BIOTECHNOLOGY, 2010, 24 (03) : 248 - 257
[38] Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
Klymus, Katy E.
Ramos, Dannise V. Ruiz
Thompson, Nathan L.
Richter, Catherine A.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2020, (165): : 1 - 25
[39] Rapid detection of Shigella species in environmental sewage by an immunocapture PCR with universal primers
Peng, XX
Luo, W
Zhang, JY
Wang, SY
Lin, SC
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (05) : 2580 - 2583
[40] FBPP: software to design PCR primers and probes for nucleic acid base detection of foodborne pathogens
Soliman, Mohamed A.
Azab, Mohamed S.
Hussein, Hala A.
Roushdy, Mohamed M.
Abu El-naga, Mohamed N.
SCIENTIFIC REPORTS, 2024, 14 (01)

← 1 2 3 4 5 →