Development of quantitative PCR primers and probes for environmental DNA detection of amphibians in Ontario

被引：9

作者：

Beauclerc, Kaela ^{[1
]}

Wozney, Kristyne ^{[2
]}

Smith, Caleigh ^{[2
]}

Wilson, Chris ^{[2
]}

机构：

[1] Trent Univ, Wildlife Res & Monitoring Sect, Ontario Minist Nat Resources & Forestry, 2140 East Bank Dr, Peterborough, ON K9L 0G2, Canada

[2] Trent Univ, Aquat Res & Monitoring Sect, Ontario Minist Nat Resources & Forestry, 2140 East Bank Dr, Peterborough, ON K9L 0G2, Canada

来源：

CONSERVATION GENETICS RESOURCES | 2019年 / 11卷 / 01期

关键词：

Amphibian; eDNA; Quantitative PCR; GREAT CRESTED NEWT; EDNA;

D O I：

10.1007/s12686-017-0962-3

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

DNA from environmental samples (eDNA) is increasingly being used to detect and monitor elusive species. We developed species-specific eDNA primers and probes for qPCR detection of 24 amphibian species native to Ontario, Canada. Cross-species testing confirmed their high specificity and low cross-species amplification, as well as their ability to detect DNA from target species at low concentrations. These detection tools should prove useful for monitoring at-risk amphibian species throughout their native ranges.

引用

页码：43 / 46

页数：4

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