Effect of culture temperature on TNFR-Fc productivity in recombinant glutamine synthetase-chinese hamster ovary cells

被引:12
|
作者
Fan, Li [1 ]
Zhao, Liang [1 ]
Ye, Zhaoyang [1 ]
Sun, Yating [1 ]
Kou, Tianci [1 ]
Zhou, Yan [1 ]
Tan, Wen-Song [1 ]
机构
[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
关键词
CHO cells; Culture temperature; Specific productivity; TNFR-Fc; FED-BATCH PROCESS; CHO-CELLS; ANTIBODY-PRODUCTION; TRANSCRIPTION LEVEL; MAMMALIAN-CELLS; PROTEIN; ERYTHROPOIETIN; CULTIVATION;
D O I
10.1007/s10529-010-0318-5
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Lowering the culture temperature is often useful to improve the production of many recombinant proteins in Chinese hamster ovary (CHO) cells. Batch cultivation of GS-CHO cells expressing TNFR-Fc antibody was therefore carried out at 30, 33.5 and 37 degrees C. TNFR-Fc productivity, qTNFR-Fc, increased as culture temperature decreased; and the maximum qTNFR-Fc was 20 mg/(10(6) cells/ml) at 30 degrees C which was three times that at 37 degrees C. Increasing the viable cell density (VCD) to above 2.2 x 10(6) cells/ml, however, decreased the qTNFR-Fc at 30 degrees C, which was due to a reduction in transcription of the TNFR-Fc gene. Taken together, lowering temperature can improve qTNFR-Fc but the negative effect of increasing VCD compromises this effect. Further process development addressing the issue of cell density-dependent TNFR-Fc productivity is therefore needed.
引用
收藏
页码:1239 / 1244
页数:6
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