共 33 条
Molecular basis of multiple resistance to herbicides inhibiting acetyl-CoA carboxylase and acetolactate synthase in American sloughgrass (Beckmannia syzigachne) from China
被引:6
|作者:
Pan, Lang
[1
,2
]
Gao, Haitao
[1
,2
]
Wu, Han
[1
,2
]
Dong, Liyao
[1
,2
]
机构:
[1] Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China
[2] Nanjing Agr Univ, Minist Agr, Key Lab Integrated Pest Management Crops Eastern, Nanjing 210095, Jiangsu, Peoples R China
来源:
基金:
中国国家自然科学基金;
关键词:
amino acid substitution;
cross-resistance;
herbicide resistance;
target-site resistance;
FENOXAPROP-P-ETHYL;
FOXTAIL ALOPECURUS-JAPONICUS;
LOLIUM-RIGIDUM POPULATION;
JAPANESE FOXTAIL;
CROSS-RESISTANCE;
ACCASE;
MUTATIONS;
STEUD;
PINOXADEN;
EVOLUTION;
D O I:
10.1071/CP16109
中图分类号:
S [农业科学];
学科分类号:
09 ;
摘要:
American sloughgrass (Beckmannia syzigachne Steud.) is a problematic grass that is widely distributed in wheat and oilseed rape fields in China. The herbicides fenoxaprop-P-ethyl and mesosulfuron-methyl failed to control B. syzigachne JCWJ-R populations collected from a wheat field in Jiangsu Province. Dose-response experiments showed that JCWJ-R was resistant to the acetyl-CoA carboxylase (ACCase) inhibitors fenoxaprop-P-ethyl (33.8-fold), haloxyfop-R-methyl (12.7-fold), clethodim (7.8-fold) and pinoxaden (11.6-fold), and to the acetolactate synthase (ALS) inhibitors mesosulfuron-methyl (15.9-fold), pyroxsulam (17.6-fold), flucarbazone-Na (10.7-fold) and imazethapyr (7-fold). Resistance to ALS inhibitors was due to a Pro-197-Ser mutation in the ALS gene and resistance to ACCase inhibitors was due to an Ile-1781-Leu mutation in the ACCase gene. A derived cleaved amplified polymorphic sequence method was developed to detect the ALS mutation in B. syzigachne. This was combined with a previously established method to detect Ile-1781-Leu, and the mutation frequency and homozygous mutation rates in the JCWJ-R population were determined. The evolution of multiple resistance to ACCase and ALS inhibitors in this B. syzigachne population indicated that alternative methods should be developed to control resistant weeds.
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页码:1208 / 1214
页数:7
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