Objective Urban green spaces provide a wide range of health benefits for the population and, therefore, their use should be promoted. However, men and women perceive and value the characteristics of urban green spaces differently, as both have different sensitivities and expectations. The present study aims to identify gender differences in the perceptions of urban green spaces characteristics. Methods Gender differences in the perceptions of eleven urban green spaces characteristics were assessed, including lighting, safety, cleanliness, walking routes, bike lanes, shaded areas, recreational areas, off-leash dog areas, children's playgrounds, drinking fountains, and pleasant views. Results Our results showed that women attribute a higher value to all the characteristics of the urban green spaces analyzed, being the most appreciated the presence of off-leash dog areas, lighting, children's playgrounds, pleasant views, drinking fountains, safety and recreational areas. In addition, older and more educated people, unemployed women, and single men consider green spaces to be highly important. Conclusion The results of this study provide evidence for the adequate planning, design and development of urban green spaces that meet the needs of all users.