Decreased E-cadherin augments β-catenin nuclear localization:: Studies in breast cancer cell lines

We showed that the YMB-1-derived breast cancer cell line YMB-S, which proliferates in suspension without aggregation, exhibits complete loss of cell-cell adhesion despite the presence of E-cadherin-catenin complex and expression of free beta -catenin in the cytoplasm. Here, we describe beta -catenin gene regulation, interaction with E-cadherin, immunocytochemical localization, and their relation to growth rate in the YMB-1-derived cell line YMB-A, which forms tight junctions and displays anchorage-dependent growth. YMB-A cells proliferated more slowly than YMB-S cells. E-cadherin and APC gene product expression in YMB-A cells was significantly higher than that in YMB-S cells, whereas expression of beta -catenin, MUC1, and c-myc was lower in YMB-A cells than in YMB-S cells. According to immunocytochemical analysis, beta -catenin in YMB-A cells displayed membranous or submembranous localization, indicating that beta -catenin is mostly tethered to E-cadherin. Inhibition of E-cadherin expression in YMB-A cells by an antisense oligonucleotide did not change expression of whole eel beta -catenin protein, but increased nuclear beta -catenin protein level, c-myc expression, and cell growth rate. These results suggest that decreased expression of E-cadherin and APC and increased amount of beta -catenin in YMB-S cells lead to accumulation of beta -catenin in the nucleus, activate beta -catenin-LEF/TCF signaling pathway, and trigger c-myc proto-oncogene expression, c-Myc overexpression in breast cancer may be related to activated Wnt independent beta -catenin-LEF/TCF signaling.