ANALYSIS OF GENETIC DIVERSITY IN NORTH INDIAN RICE (ORYZA SATIVA L.) GERMPLASM, USING SSR AND ISSR MARKERS

Genetic diversity among thirty two accessions of rice representing landraces, released varieties, cultivars and breeding lines was analysed using simple sequence repeat (SSR), inter-simple sequence repeat (ISSR) and three dwarfing trait based markers. A total of 81 polymorphic bands were detected with 20 primer combinations. The genetic similarity among the accessions ranged from (0.60) to (0.97) with a mean of 0.737. All 32 accessions were clustered into 5 major groups. Of the 32 pair wise combinations generated by rice genotypes, the lowest coefficient of similarity 0.505 was observed between Sarbati-A and Sathi, Bagni and VB24 genotypes. The highest coefficient of similarity 0.964 was obtained between VD 62 and VLD86, which appear as the most similar and closely related accessions. Results illustrated the high-level polymorphism obtained by SSR and ISSR, especially trait-linked markers used in this study. These markers will applicable in discriminating different germplasm, and utility of these markers could be deployable in hybrid breeding programs, as well as in marker assisted selection (MAS).