A study of the effect on human mesenchymal stem cells of an atmospheric pressure plasma source driven by different voltage waveforms

被引：5

作者：

Laurita, R. ^{[1
]}

Alviano, F. ^{[2
]}

Marchionni, C. ^{[2
]}

Abruzzo, P. M. ^{[2
]}

Bolotta, A. ^{[2
]}

Bonsi, L. ^{[2
]}

Colombo, V. ^{[1
,5
]}

Gherardi, M. ^{[1
,5
]}

Liguori, A. ^{[1
]}

Ricci, F. ^{[3
]}

Rossi, M. ^{[2
]}

Stancampiano, A. ^{[1
]}

Tazzari, P. L. ^{[3
]}

Marini, M. ^{[2
,4
]}

机构：

[1] Alma Mater Studiorum Univ Bologna, Dept Ind Engn DIN, Via Saragozza 8, I-40123 Bologna, Italy

[2] Alma Mater Studiorum Univ Bologna, Dept Expt Diagnost & Specialty Med DIMES, Via Belmeloro 8, I-40126 Bologna, Italy

[3] St Orsola Marcello Malpighi Hosp, Immunohaematol & Transfus Med Serv, Via Massarenti 9, I-40138 Bologna, Italy

[4] IRCCS S Maria Nascente, Don Gnocchi Fdn ONLUS, Via Alfonso Capecelatro 66, I-20148 Milan, Italy

[5] Alma Mater Studiorum Univ Bologna, Ind Res Ctr Adv Mech & Mat CIRIMAM, Via Saragozza 8, I-40123 Bologna, Italy

来源：

JOURNAL OF PHYSICS D-APPLIED PHYSICS | 2016年 / 49卷 / 36期

关键词：

atmospheric pressure non-equilibrium plasma; dielectric barrier discharge; high voltage pulsed discharge; fetal membrane-derived human mesenchymal stem cells; LOW-TEMPERATURE PLASMA; ELECTRICAL PULSES; ARGON PLASMA; CANCER; ELECTROPORATION; SENESCENCE; STRATEGY; CYCLE; STATE;

D O I：

10.1088/0022-3727/49/36/364003

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

The effect of an atmospheric pressure non-equilibrium plasma on human mesenchymal stem cells was investigated. A dielectric barrier discharge non-equilibrium plasma source driven by two different high-voltage pulsed generators was used and cell survival, senescence, proliferation, and differentiation were evaluated. Cells deprived of the culture medium and treated with nanosecond pulsed plasma showed a higher mortality rate, while higher survival and retention of proliferation were observed in cells treated with microsecond pulsed plasma in the presence of the culture medium. While a few treated cells showed the hallmarks of senescence, unexpected delayed apoptosis ensued in cells exposed to plasma-treated medium. The plasma treatment did not change the expression of OCT4, a marker of mesenchymal stem cell differentiation.

引用

页数：14

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