Spectrofluorometric Determination of Total Bilirubin in Human Serum Samples Using Tetracycline-Eu3+

被引：11
|
作者：
Bian, Weiwei [1 ]
Zhang, Na [2 ]
Wang, Le [3 ]
机构：
[1] Weifang Med Univ, Weifang 261053, Shandong, Peoples R China
[2] Huangshan Univ, Dept Chem, Huangshan 245041, Peoples R China
[3] Zibo Entry & Exit Inspect & Quarantine Bur, State Key Lab Ceram, Zibo 255031, Peoples R China
来源：
ANALYTICAL SCIENCES | 2010年 / 26卷 / 07期
关键词：
CAPILLARY-ELECTROPHORESIS; CHEMILUMINESCENCE; ALBUMIN; ASSAY;
D O I：
10.2116/analsci.26.785
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The measurement of total bilirubin in serum is one of the most frequently performed tests in clinical analysis. A new spectrofluorometric method was developed for the determination of trace amounts of total bilirubin. Using tetracycline-Eu3+ as a fluorescent probe, bilirubin can remarkably reduce the fluorescence intensity of the tetracycline-Eu3+ complex, and the reduced fluorescence intensity of Eu3+ ion is in proportion to the concentration of bilirubin. The optimum conditions for the determination of bilirubin were also investigated. This method is simple, practical, and relatively free of interference from coexisting substances. It can be successfully applied to assess total bilirubin in serum samples. We also compared the developed method with a modified Jendrassik-Grof method in clinical analysis.
引用
收藏
页码：785 / 789
页数：5
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