In utero exposure to triphenyltin disrupts rat fetal testis development

Triphenyltin is an organotin that is widely used as an anti-fouling agent and may have endocrine disrupting effects. The objective of the current study was to investigate effects of triphenyltin on the development of rat fetal testis. Female pregnant Sprague Dawley dams were gavaged daily with triphenyltin (0, 0.5, 1, and 2 mg/kg body weight/day) from gestational day 12 to day 21. Triphenyltin dose dependently decreased serum testosterone levels (0.971 +/- 0.072 and 0.972 +/- 0.231 ng/ml at 1 and 2 mg/kg, respectively) from control level (2.099 +/- 0.351 ng/ml). Triphenyltin at 1 and 2 mg/kg doses also induced fetal Leydig cell aggregation, decreased fetal Leydig cell size and cytoplasmic size. Triphenyltin decreased the expression levels of Lhcgr, Scarb1, Star, Cyp11a1, Cyp17a1, Insl3, Fshr, Pdgfa, and Sox9 by 0.5 mg/kg dose and above. However, triphenyltin did not affect Leydig and Sertoli cell numbers. In conclusion, the current study indicated that in utero exposure of triphenyltin disrupted fetal Leydig and Sertoli cell development. (C) 2018 Elsevier Ltd. All rights reserved.