A non-radioisotopic anion-exchange chromatographic method to measure the CO2/O-2 specificity factor for ribulose bisphosphate carboxylase/oxygenase

被引：6

作者：

Yaguchi, T

Oguni, A

Ouchiyama, N

Igarashi, Y

Kodama, T

机构：

[1] SUNTORY LTD, INST FUNDAMENTAL RES, SHIMAMOTO, OSAKA 618, JAPAN

[2] CHEM INSPECT & TESTING INST, KURUME RES LABS, FUKUOKA 830, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1996年 / 60卷 / 06期

关键词：

ribulose 1,5-bisphosphate carboxylase/oxygenase; RubisCO; specificity factor; enzyme assay;

D O I：

10.1271/bbb.60.942

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A non-radioisotopic anion-exchange ion chromatographic method for measuring the carboxylation/oxygenation specificity (tau) of ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) is presented. The assay measures the amounts of fixation products at varying [CO2]/[O-2] ratios to measure the relative rates of CO2 and O-2 fixation reactions, The amount of 3-phosphoglycerate (3-PGA) and phosphoglycolate (PG) in the reaction mixture were measured with a conductivity detector and the specific factor was calculated using the following equations: v(c) = ([3-PGA] - [PG])/2 and v(0) = [PG]. By this method, specificity factors for RubisCOs were measured without using radioactive reagents.

引用

页码：942 / 944

页数：3

共 50 条

[21] EFFECTS OF PHOSPHORUS-NUTRITION ON THE RESPONSE OF PHOTOSYNTHESIS TO CO2 AND O-2, ACTIVATION OF RIBULOSE BISPHOSPHATE CARBOXYLASE AND AMOUNTS OF RIBULOSE BISPHOSPHATE AND 3-PHOSPHOGLYCERATE IN SPINACH LEAVES
BROOKS, A
WOO, KC
WONG, SC
PHOTOSYNTHESIS RESEARCH, 1988, 15 (02) : 133 - 141
[22] Measurements of the CO2/O2 specificity of ribulose 1,5-bisphosphate carboxylase/oxygenase by 31P- and 1H-NMR
Dept. of Biomolecular Engineering, Center for Interdisciplinary Science, Tohoku University, Sendai 980-8579, Japan
Photosynth. Res., 1 (103-109):
[23] Measurements of the CO2/O2 specificity of ribulose 1, 5-bisphosphate carboxylase/oxygenase by 31P- and 1H-NMR
Zheng-Yu Wang
Shen Luo
Kazuhiro Sato
Masayuki Kobayashi
Tsunenori Nozawa
Photosynthesis Research, 1998, 58 : 103 - 109
[24] Substitutions at methionine 295 of Archaeoglobus fulgidus ribulose-1,5-bisphosphate carboxylase/oxygenase affect oxygen binding and CO2/O2 specificity
Kreel, Nathaniel E.
Tabita, F. Robert
JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (02) : 1341 - 1351
[25] Measurements of the CO2/O2 specificity of ribulose 1,5-bisphosphate carboxylase/oxygenase by 31P- and 1H-NMR
Wang, ZY
Luo, S
Sato, K
Kobayashi, M
Nozawa, T
PHOTOSYNTHESIS RESEARCH, 1998, 58 (01) : 103 - 109
[26] FORMATION OF STOICHIOMETRIC COBALT(III)-CO2 COMPLEXES WITH SPINACH RIBULOSE BISPHOSPHATE CARBOXYLASE-OXYGENASE
ROBISON, PD
TABITA, FR
FEBS LETTERS, 1980, 111 (02) : 369 - 372
[27] EXTREME PHOSPHATE DEFICIENCY DECREASES THE IN-VIVO CO2/O2 SPECIFICITY FACTOR OF RIBULOSE 1,5-BISPHOSPHATE CARBOXYLASE-OXYGENASE IN INTACT LEAVES OF SUNFLOWER
JACOB, J
LAWLOR, DW
JOURNAL OF EXPERIMENTAL BOTANY, 1993, 44 (268) : 1635 - 1641
[28] ACTIVATION AND CO2 EXCHANGE KINETICS OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE OXYGENASE - NEGATIVE COOPERATIVITY WITH RESPECT TO MG-2+
BELKNAP, WR
PORTIS, AR
BIOCHEMISTRY, 1986, 25 (08) : 1864 - 1869
[29] EFFECT OF TEMPERATURE ON THE CO2/O2 SPECIFICITY OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE OXYGENASE AND THE RATE OF RESPIRATION IN THE LIGHT - ESTIMATES FROM GAS-EXCHANGE MEASUREMENTS ON SPINACH
BROOKS, A
FARQUHAR, GD
PLANTA, 1985, 165 (03) : 397 - 406
[30] EFFECTS OF ILLUMINATION UNDER LOW CO2 AND O-2 LEVELS ON PHOTOSYNTHESIS, RIBULOSE BISPHOSPHATE CARBOXYLASE ACTIVITY AND RuBP LEVELS IN WHEAT
Gustafson, Steven W.
Raynes, Deborah A.
Jensen, Richard G.
PLANT PHYSIOLOGY, 1984, 75 : 6 - 6

← 1 2 3 4 5 →