Supercritical carbon dioxide extraction of ethyl p-methoxycinnamate from Kaempferia galanga L. rhizome and its apoptotic induction in human HepG2 cells

被引:31
|
作者
Liu, Benguo [2 ]
Liu, Feng [1 ]
Chen, Chungang [2 ]
Gao, Han [2 ]
机构
[1] Tsinghua Univ, Grad Sch Shenzhen, Key Lab Chem Biol, Shenzhen 518055, Guangdong, Peoples R China
[2] Henan Inst Sci & Technol, Sch Food Sci, Xinxiang 453003, Peoples R China
关键词
ethyl p-methoxycinnamate; supercritical carbon dioxide extraction; Kaempferia galanga L; apoptosis; cell cycle; HepG2; PATHOGENESIS; ANTIOXIDANT; INHIBITION; TOXICITY; LEAVES; DEATH; ASSAY;
D O I
10.1080/14786419.2010.490913
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
In this study, supercritical carbon dioxide extraction of ethyl p-methoxycinnamate from Kaempferia galanga L. rhizome and its apoptotic induction in human HepG2 cells are reported for the first time. By using supercritical carbon dioxide extraction, the yield of ethyl p-methoxycinnamate identified by gas chromatography mass spectrometry (GC-MS) was as high as 2.5% with respect to the raw materials. In the anticancer assay, it was found that ethyl p-methoxycinnamate could inhibit the proliferation of the human hepatocellular liver carcinoma HepG2 cell line in a dose-dependent manner and induce the significant increase of the subG0 cell population. After treatment with ethyl p-methoxycinnamate, phosphatidylserine of HepG2 cells could significantly translocate to the surface of the membrane. The increase of an early apoptotic population was observed by both annexin-fluorescein isothiocyanate (FITC) and propidium iodide (PI) staining. It was concluded that ethyl p-methoxycinnamate not only induced cells to enter into apoptosis, but also affected the progress of the cell cycle.
引用
收藏
页码:1927 / 1932
页数:6
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